Stem Cells (Human Embryonic) and Cancer
Published Online: 15 SEP 2006
Copyright © 2006 Wiley-VCH Verlag GmbH & Co. KGaA. All rights reserved.
Reviews in Cell Biology and Molecular Medicine
How to Cite
Tzukerman, M. and Skorecki, K. 2006. Stem Cells (Human Embryonic) and Cancer. Reviews in Cell Biology and Molecular Medicine. .
- Published Online: 15 SEP 2006
There is currently no experimental system available wherein human cancer cells can be grown in the context of a mixed population of normal differentiated human cells for testing biological aspects of cancer cell growth (tumor cell invasion, angiogenesis), or response to anticancer therapies. Human embryonic stem cells when implanted into immunocompromised mice develop teratomas containing complex structures, comprising differentiated cell types representing the major germ-line derived lineages. We sought to determine whether human cancer cells would grow within such teratomas and display properties associated with malignancy, such as invasiveness and recruitment of blood vessels. Ovarian cancer cells (HEY), stably expressing an H2A-GFP fusion protein, which allows tracking of tumor cells, were injected into mature teratomas and developed into tumors. The growth, proliferative capacity, invasion, and induction of blood vessel formation were examined. We propose using this novel experimental platform to develop a preclinical model for investigating and manipulating the stromal response in tumor cell growth as an additional tool in cancer research.
- Human embryonic stem cells;
- Immunodeficient SCID/beige mouse;
- Tumor microenvironment;