Standard Article


Systems Biology

  1. Teresa M. Przytycka,
  2. Dong-Yeon Cho

Published Online: 15 MAY 2012

DOI: 10.1002/3527600906.mcb.201100018

Reviews in Cell Biology and Molecular Medicine

Reviews in Cell Biology and Molecular Medicine

How to Cite

Przytycka, T. M. and Cho, D.-Y. 2012. Interactome. Reviews in Cell Biology and Molecular Medicine. .

Author Information

  1. National Institute of Health (NIH), National Center of Biotechnology Information, NLM, Bethesda, MD, USA

Publication History

  1. Published Online: 15 MAY 2012


The interactome of a cell consists of all the molecular interactions that occur within that cell. Among the diverse types of molecular interaction, genome-wide protein–protein interactions are the most broadly studied. In this chapter, attention is focused on genome-scale methods to infer and to study protein–protein interactions. Following a brief introduction to the subject, the experimental and computational techniques used to uncover interactions between proteins are outlined. In the context of computational approaches, methods are also discussed for predicting interactions between protein domains. Later, the basic topological properties of protein interaction networks are described, such as node degree distribution, modularity, and network motifs. The motivation and approaches for comparing biological networks are then detailed, followed by a brief introduction to protein and domain interaction databases. Finally, some sample applications are provided that take advantage of interactome data, and details are proposed for the possible future direction of interactom e-related research.


  • Coevolution;
  • Coexpression;
  • Graph/network;
  • Gene regulatory network;
  • Homologs and orthologs;
  • Protein–protein interaction network;
  • Transcription factor;
  • Yeast tw o-hybrid (Y2H)