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Cloned Mice from Embryonic Stem Cells

  1. Chong Li,
  2. Teruhiko Wakayama

Published Online: 15 JUL 2012

DOI: 10.1002/3527600906.mcb.201100022

Encyclopedia of Molecular Cell Biology and Molecular Medicine

Encyclopedia of Molecular Cell Biology and Molecular Medicine

How to Cite

Li, C. and Wakayama, T. 2012. Cloned Mice from Embryonic Stem Cells. Encyclopedia of Molecular Cell Biology and Molecular Medicine. .

Author Information

  1. Laboratory for Genomic Reprogramming, RIKEN, Center for Developmental Biology, Kobe, Japan

Publication History

  1. Published Online: 15 JUL 2012

Abstract

Mouse embryonic stem (ES) cells can be used as donors to generate cloned mice via the nuclear transfer (NT) technique. It has been shown that NT-derived ES (ntES) cell lines can be established from somatic cell nuclei with a 10-fold higher success rate than the production rate of cloned mice. The ntES cell technology can be used to generate cloned animals from ntES cell lines, using a second round of NT. Recently, a description was provided of how healthy offspring can be generated from infertile mice strains, from the nuclei of cells from dead bodies, or from freeze-dried cells by cloning using ntES cell technology. Currently, only the ntES cell technology is available for this purpose. Although ethical problems with ntES cell technology remain, it has the emerging potential to become a powerful tool for broad-based applications in the study of basic biology.

Keywords:

  • Clone;
  • Nuclear transfer;
  • Reprogramming;
  • ntES cell