Cloned Mice from Embryonic Stem Cells
Published Online: 15 JUL 2012
Copyright © 2012 Wiley-VCH Verlag GmbH & Co. KGaA
Encyclopedia of Molecular Cell Biology and Molecular Medicine
How to Cite
Li, C. and Wakayama, T. 2012. Cloned Mice from Embryonic Stem Cells. Encyclopedia of Molecular Cell Biology and Molecular Medicine. .
- Published Online: 15 JUL 2012
Mouse embryonic stem (ES) cells can be used as donors to generate cloned mice via the nuclear transfer (NT) technique. It has been shown that NT-derived ES (ntES) cell lines can be established from somatic cell nuclei with a 10-fold higher success rate than the production rate of cloned mice. The ntES cell technology can be used to generate cloned animals from ntES cell lines, using a second round of NT. Recently, a description was provided of how healthy offspring can be generated from infertile mice strains, from the nuclei of cells from dead bodies, or from freeze-dried cells by cloning using ntES cell technology. Currently, only the ntES cell technology is available for this purpose. Although ethical problems with ntES cell technology remain, it has the emerging potential to become a powerful tool for broad-based applications in the study of basic biology.
- Nuclear transfer;
- ntES cell