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DNA Methylation Analysis by MALDI Mass Spectrometry

Epigenetic Regulation and Epigenomics

  1. Jörg Tost1,2,
  2. Ivo G. Gut3

Published Online: 15 MAR 2012

DOI: 10.1002/3527600906.mcb.201100025

Reviews in Cell Biology and Molecular Medicine

Reviews in Cell Biology and Molecular Medicine

How to Cite

Tost, J. and Gut, I. G. 2012. DNA Methylation Analysis by MALDI Mass Spectrometry. Reviews in Cell Biology and Molecular Medicine. .

Author Information

  1. 1

    Centre National de Génotypage, CEA-Institut de Genomique, Laboratory for Epigenetics, Evry, France

  2. 2

    Fondation Jean Dausset - CEPH, Laboratory for Functional Genomics, Paris, France

  3. 3

    Centro Nacional de Analisis Genomico, Barcelona, Spain

Publication History

  1. Published Online: 15 MAR 2012

Abstract

Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has emerged as a very powerful method for the analysis of nucleic acids, in particular for the genotyping of single nucleotide polymorphisms. The accuracy, speed of data accumulation, and data structure are the major advantages of MALDI-TOF mass spectrometric analysis. Other applications of MALDI-TOF MS are de novo sequencing, re-sequencing, and DNA methylation analysis. In this chapter, the methods developed for the application of MALDI-TOF MS for DNA methylation are described, together with some of the major applications of these methods in research and clinically oriented projects.

Keywords:

  • DNA methylation;
  • DNA;
  • Mass spectrometry;
  • MALDI