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Tag Sequencing

Epigenetic Regulation and Epigenomics

  1. Keerthana Krishnan,
  2. David L. A. Wood,
  3. Jason A. Steen,
  4. Sean M. Grimmond,
  5. Nicole Cloonan

Published Online: 15 MAY 2012

DOI: 10.1002/3527600906.mcb.201100033

Reviews in Cell Biology and Molecular Medicine

Reviews in Cell Biology and Molecular Medicine

How to Cite

Krishnan, K., Wood, D. L. A., Steen, J. A., Grimmond, S. M. and Cloonan, N. 2012. Tag Sequencing. Reviews in Cell Biology and Molecular Medicine. .

Author Information

  1. The University of Queensland, Queensland Centre for Medical Genomics, Institute for Molecular Bioscience, St Lucia, Queensland, Australia

Publication History

  1. Published Online: 15 MAY 2012

Abstract

Currently, technological advances in sequencing are revolutionizing the way in which genome-scale biological research is performed. This is leading to an “explosion” of applications, made possible by the massive scale of data generated, and a similarly massive decrease in costs. Although any specific mention of throughputs, lengths, or costs will quickly become outdated as the technology is still rapidly evolving, the concepts and protocols surrounding the technology have largely stabilized. In this chapter, the technology, analysis, and common applications of tag sequencing will be explored, and an idea provided of how these will continue to impact on the major facets of nucleic acid research, namely genomics, epigenomics, and transcriptomics.

Keywords:

  • Tag sequencing;
  • PCR: Polymerase chain reaction;
  • Library;
  • Genome;
  • Transcriptome;
  • Epigenome