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Induction of Pluripotent Stem Cells from Umbilical Cord Blood

  1. Kejin Hu1,
  2. Igor Slukvin2

Published Online: 30 NOV 2012

DOI: 10.1002/3527600906.mcb.201200006

Encyclopedia of Molecular Cell Biology and Molecular Medicine

Encyclopedia of Molecular Cell Biology and Molecular Medicine

How to Cite

Hu, K. and Slukvin, I. 2012. Induction of Pluripotent Stem Cells from Umbilical Cord Blood. Encyclopedia of Molecular Cell Biology and Molecular Medicine. .

Author Information

  1. 1

    Univesrsity of Alabama at Birmingham, UAB Stem Cell Institute, Department of Biochemistry and Molecular Genetics, School of Medicine, Birmingham, AB, USA

  2. 2

    University of Wisconsin-Madison, Department of Pathology and Laboratory Medicine, Wisconsin National Primate Research Center, Madison, WI, USA

Publication History

  1. Published Online: 30 NOV 2012

Abstract

Human induced pluripotent stem cells (hiPSCs) have become an important tool for the studies of human cell specification, disease modeling, and drug screening. They also hold promise for regenerative medicine as a potential source of patient-specific donor cells. Human cord blood (CB) represents one of the most accessible sources of somatic cells, with extensive and diverse archived samples available. The successful reprogramming of umbilical CB cells stored for more than 20 years suggests a minimal effect of storage on their properties, and opens these stocks for future exploration. Although isolated stem cells/progenitors and the viral delivery of reprogramming factors were utilized in the initial CB reprogramming methods, recent studies have demonstrated the efficient generation of hiPSCs free of exogenous sequences from just 1 ml of CB, using the unfractionated total mononuclear cells and the non-integrating Epstein–Barr virus-based episomal vectors with only one round of transfection. Advancing CB-derived hiPSCs towards clinical applications will require the development of completely defined conditions for hiPSC derivation and differentiation, and an evaluation of the therapeutic efficiency and safety of these cells in animal models.

Keywords:

  • Pluripotent stem cells;
  • Embryonic stem cells;
  • Induced pluripotent stem cells;
  • Umbilical cord blood