Chapter 2. Sample Preparation and Supplementary Tools

  1. Dr. Stefan Lorkowski7 and
  2. Dr. Paul Cullen8
  1. Dr. Stefan Lorkowski7,*,
  2. Dr. Paul Cullen8,
  3. Giovanni Salerno1,
  4. Renate Burgemeister2,
  5. Karin Schütze2,
  6. Douglas J. McGarvey3,*,
  7. Achim Quandt3 and
  8. Jörg Haberland4

Published Online: 27 MAY 2004

DOI: 10.1002/352760149X.ch2

Analysing Gene Expression: A Handbook of Methods: Possibilities and Pitfalls

Analysing Gene Expression: A Handbook of Methods: Possibilities and Pitfalls

How to Cite

Lorkowski, S., Cullen, P., Salerno, G., Burgemeister, R., Schütze, K., McGarvey, D. J., Quandt, A. and Haberland, J. (2002) Sample Preparation and Supplementary Tools, in Analysing Gene Expression: A Handbook of Methods: Possibilities and Pitfalls (eds S. Lorkowski and P. Cullen), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/352760149X.ch2

Editor Information

  1. 7

    Institute of Arteriosclerosis Research, University of Münster, Domagkstr. 3, 48149 Münster, Germany

  2. 8

    Ogham GmbH, Mendelstr. 11, 48149 Münster, Germany

Author Information

  1. 1

    DakoCytomation Flow Center GmbH, Freiburg i. Br. (Germany)

  2. 2

    P.A.L.M. Microlaser Technologies AG, Bernried (Germany)

  3. 3

    QIAGEN GmbH, Hilden (Germany)

  4. 4

    Ogham GmbH, Münster (Germany)

  5. 7

    Institute of Arteriosclerosis Research, University of Münster, Domagkstr. 3, 48149 Münster, Germany

  6. 8

    Ogham GmbH, Mendelstr. 11, 48149 Münster, Germany

*Institute of Arteriosclerosis Research, University of Münster, Domagkstr. 3, 48149 Münster, Germany

Publication History

  1. Published Online: 27 MAY 2004
  2. Published Print: 1 DEC 2002

ISBN Information

Print ISBN: 9783527304882

Online ISBN: 9783527601493

SEARCH

Keywords:

  • sample preparation;
  • cell isolation and culturing techniques;
  • immunopurification;
  • centrifugations;
  • surface affinity chromatography;
  • flow cytometry;
  • tissue microdissection techniques;
  • nucleic acids and proteins;
  • isolation of total RNA and mRNA;
  • stabilisation reagents;
  • preparation of protein samples

Summary

This chapter contains sections titled:

  • Introduction

  • Preparation of cells and tissues

    • Immunopurification of cells

    • Differential-velocity centrifugation/countercurrent elutriation

    • Surface affinity chromatography

    • Density-gradient centrifugation

    • Flow cytometry

    • Tissue microdissection techniques

    • Miscellaneous cell isolation and culturing techniques

  • Preparation of nucleic acids and proteins

    • Introduction

    • Isolation of total RNA and mRNA

    • Stabilisation of RNA prior to isolation

    • Preparation of protein samples from cell cultures and tissues

  • Summary

  • References