Chapter 6. Methods for mRNA and Protein Expression Analysis in situ and in vivo

  1. Dr. Stefan Lorkowski9 and
  2. Dr. Paul Cullen10
  1. Dr. Paul Cullen10,*,
  2. Dr. Stefan Lorkowski9,*,
  3. Clive N. Kind1,
  4. Michael Schnoor2,
  5. Christoph Bremer2,
  6. Ralph Weissleder3,*,
  7. Heiko Alfke4,*,
  8. Johannes T. Heverhagen4,
  9. Thomas M. Behr4,* and
  10. Martin P. Béhé4

Published Online: 27 MAY 2004

DOI: 10.1002/352760149X.ch6

Analysing Gene Expression: A Handbook of Methods: Possibilities and Pitfalls

Analysing Gene Expression: A Handbook of Methods: Possibilities and Pitfalls

How to Cite

Cullen, P., Lorkowski, S., Kind, C. N., Schnoor, M., Bremer, C., Weissleder, R., Alfke, H., Heverhagen, J. T., Behr, T. M. and Béhé, M. P. (2002) Methods for mRNA and Protein Expression Analysis in situ and in vivo, in Analysing Gene Expression: A Handbook of Methods: Possibilities and Pitfalls (eds S. Lorkowski and P. Cullen), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/352760149X.ch6

Editor Information

  1. 9

    Institute of Arteriosclerosis Research, University of Münster, Domagkstr. 3, 48149 Münster, Germany

  2. 10

    Ogham GmbH, Mendelstr. 11, 48149 Münster, Germany

Author Information

  1. 1

    AstraZeneca R & D Charnwood, Loughborough (UK)

  2. 2

    University of Münster, Münster (Germany)

  3. 3

    Massachusetts General Hospital, Charlestown (USA)

  4. 4

    Philipps University, Marburg (Germany)

  5. 9

    Institute of Arteriosclerosis Research, University of Münster, Domagkstr. 3, 48149 Münster, Germany

  6. 10

    Ogham GmbH, Mendelstr. 11, 48149 Münster, Germany

*Ogham GmbH, Mendelstr. 11, 48149 Münster, Germany

Publication History

  1. Published Online: 27 MAY 2004
  2. Published Print: 1 DEC 2002

ISBN Information

Print ISBN: 9783527304882

Online ISBN: 9783527601493

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Keywords:

  • mRNA and protein expression;
  • methods for analysis;
  • in situ hybridisation;
  • immunocytochemistry;
  • immunohistochemistry;
  • visualising RNA

Summary

This chapter contains sections titled:

  • Introduction

  • In situ hybridisation, immunocytochemistry and immunohistochemistry

    • Introduction

    • In situ hybridisation

    • Immunocytochemistry and immunohistochemistry

    • Advanced techniques

    • Application in the study of drug efficacy and toxicity

  • Visualising RNA in living cells

    • Introduction

    • Fluorescence in vivo hybridisation

    • Alternative methods for visualising RNA in vivo

  • MRI – Magnetic resonance imaging

    • Introduction

    • Imaging mRNA with magnetic switches

    • Imaging proteins with targeted probes

    • High-throughput imaging

    • Magnetic resonance-sensitive marker genes

  • MRS – Magnetic resonance spectroscopy

    • Introduction

    • Principles and basics

    • In vivo spectroscopy of mRNA and protein expression

    • Discussion

  • PET – Positron emission tomography

    • Introduction

    • From clinical PET to quantitative small laboratory animal tomography

    • Examples of molecular imaging and imaging of gene expression with PET

    • Quantitative imaging in oncology

    • Quantitative imaging of transgene and endogenous gene expression

    • Discussion and conclusions

  • SPECT – Single photon emission computed tomography

    • Introduction

    • Principles and basics

    • Direct imaging of gene expression

    • Indirect imaging via expressed proteins

    • Conclusions

  • Optical in vivo imaging

    • Introduction

    • Fluorescent proteins

    • Bioluminescence

    • NIRF – Near-infrared fluorescent imaging

    • Enzyme-sensing, activatable NIRF probes

  • Summary

  • References