Chapter 3. Epi Meets Genomics: Technologies for Finding and Reading the 5th Base

  1. Dr. Stephan Beck1,
  2. Dr. Alexander Olek2
  1. Tim Hui-Ming Huang,
  2. Christoph Plass,
  3. Gangning Liang,
  4. Peter W. Laird

Published Online: 16 MAR 2005

DOI: 10.1002/3527601511.ch3

Book Title

The Epigenome: Molecular Hide and Seek

The Epigenome: Molecular Hide and Seek

Additional Information

How to Cite

Huang, T. H.-M., Plass, C., Liang, G. and Laird, P. W. (2005) Epi Meets Genomics: Technologies for Finding and Reading the 5th Base, in The Epigenome: Molecular Hide and Seek (eds S. Beck and A. Olek), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527601511.ch3

Editor Information

  1. 1

    The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB 10 1SA, UK

  2. 2

    Epigenomics AG, Kastanienallee 24, 10435 Berlin, Germany

Publication History

  1. Published Online: 16 MAR 2005
  2. Published Print: 21 FEB 2003

ISBN Information

Print ISBN: 9783527304943

Online ISBN: 9783527601516

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CHAPTER TOOLS

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Keywords:

  • 5th base;
  • DNA-cutting enzymes;
  • restriction landmark genomic scanning;
  • arbitrarily primed PCR;
  • genomic ponds;
  • differential methylation hybridization;
  • genomic shadows;
  • MethyLight;
  • epigenome

Summary

This chapter contains sections titled:

  • The Development of 5th-Base Technologies

    • Unusual DNA-cutting Enzymes

    • A Unique Chemical Reaction that Modifies Methylated DNA

    • The Advance of 5th Base Technologies in Epigenomic Research

  • Restriction Landmark Genomic Scanning (RLGS): Finding the 5th-base Signposts in the Genomic Atlas

    • Principle

    • How Does RLGS Work?

    • Applications

  • Methylation-sensitive Arbitrarily Primed (AP) PCR: Fishing for the 5th Bases in Genomic Ponds

    • Principle

    • How Does MS AP-PCR Work?

    • Applications

  • Differential Methylation Hybridization (DMH): Identifying the 5th Bases in the Genomic Crossword Puzzle

    • Principle

    • How Does DMH Work?

    • Applications

  • MethyLight: Finding 5th-base Patterns in Genomic Shadows

    • Principle

    • How Does MethyLight Work?

    • Applications

  • Exploring the Epigenome

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