Chapter 18. Human Cerebrospinal Fluid
- Dr. Jean-Charles Sanchez,
- Dr. Garry L. Corthals,
- Prof. Dr. Denis F. Hochstrasser
Published Online: 20 SEP 2004
DOI: 10.1002/3527601562.ch18
Copyright © 2004 Wiley-VCH Verlag GmbH & Co. KGaA
Book Title
Biomedical Applications of Proteomics
Additional Information
How to Cite
Davidsson, P. and Harrington, M. G. (2004) Human Cerebrospinal Fluid, in Biomedical Applications of Proteomics (eds J.-C. Sanchez, G. L. Corthals and D. F. Hochstrasser), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527601562.ch18
Editor Information
Biomedical Proteomics Research Group, Laboratoire Central de Chimie Clinique, Hopitaux Universitaires de Genève, 24, rue Micheli-du-Crest, 1211 Genève 14, Switzerland
Publication History
- Published Online: 20 SEP 2004
- Published Print: 27 JAN 2004
ISBN Information
Print ISBN: 9783527308071
Online ISBN: 9783527601561
- Summary
- Chapter
Keywords:
- cerebrospinal fluid;
- experimental design;
- CSF proteomic applications;
- central nervous system diseases
Summary
Introduction
Experimental Design
Sample Collection and Preparation
Two-dimensional Gels of CSF, with Protein Identification by Antibodies, Edman Degradation Chemistry, MALDI/MS or LC/MS of Individual “Spots”
Prefractionation of CSF Prior to 2-DE
Two-dimensional Liquid-phase Electrophoresis and Other LC-coupled MS Approaches
CSF Proteomic Applications in Central Nervous System Diseases
Future Challenges
References