Chapter 7. Proteomic Approaches for Biomarker Discovery in Colorectal Cancer

  1. Dr. Jean-Charles Sanchez,
  2. Dr. Garry L. Corthals,
  3. Prof. Dr. Denis F. Hochstrasser
  1. Prof. Dr. Richard J. Simpson1,
  2. Dr. Donna S. Dorow2

Published Online: 20 SEP 2004

DOI: 10.1002/3527601562.ch7

Book Title

Biomedical Applications of Proteomics

Biomedical Applications of Proteomics

Additional Information

How to Cite

Simpson, R. J. and Dorow, D. S. (2004) Proteomic Approaches for Biomarker Discovery in Colorectal Cancer, in Biomedical Applications of Proteomics (eds J.-C. Sanchez, G. L. Corthals and D. F. Hochstrasser), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527601562.ch7

Editor Information

  1. Biomedical Proteomics Research Group, Laboratoire Central de Chimie Clinique, Hopitaux Universitaires de Genève, 24, rue Micheli-du-Crest, 1211 Genève 14, Switzerland

Author Information

  1. 1

    Joint Proteomic Structure Laboratory, Ludwig Institute for Cancer Research, Royal Melbourne Hospital, Post Office Box 2008, 3050 Melbourne, Victoria, Australia

  2. 2

    Center for Molecular Imaging & Trescowthick Research Laboratories, Peter MacCallum Cancer Institute, Melbourne 8006, Victoria, Australia

Publication History

  1. Published Online: 20 SEP 2004
  2. Published Print: 27 JAN 2004

ISBN Information

Print ISBN: 9783527308071

Online ISBN: 9783527601561

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Keywords:

  • colorectal cancer;
  • biomarker discovery;
  • ELISA;
  • gel electrophoresis;
  • SDS-PAGE;
  • proteomic pattern diagnostics;
  • microarrays;
  • tumor-specific autoantigens

Summary

  • Introduction and Background to Colorectal Cancer

  • Molecular Basis of Colorectal Cancer: Tumorigenesis is a Multistep Process

  • The Case for Early Detection: CRC is Treatable if Detected Early

  • Approaches to Biomarker Discovery

    • Use of ELISAs to Detect CRC

    • Two-dimensional Gel Electrophoresis

    • One-dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) Plasma Membrane Proteome

    • Multidimensional Protein Identification Technologies

    • Proteomic Pattern Diagnostics

    • Protein Microarrays

    • Proteomic Approaches for Identifying Tumor-specific Autoantigens

  • Conclusions

  • References

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