Chapter 4. Practical Applications of Biocatalysis for the Manufacture of Chiral Alcohols Such as (R)-1,3-Butanediol by Stereospecific Oxidoreduction
- Dr. Hans-Ulrich Blaser2,
- Dr. Elke Schmidt3
Published Online: 13 JAN 2004
DOI: 10.1002/3527602151.ch12
Copyright © 2004 Wiley-VCH Verlag GmbH & Co. KGaA
Book Title
Asymmetric Catalysis on Industrial Scale: Challenges, Approaches and Solutions
Additional Information
How to Cite
Matsuyama, A. and Yamamoto, H. (2004) Practical Applications of Biocatalysis for the Manufacture of Chiral Alcohols Such as (R)-1,3-Butanediol by Stereospecific Oxidoreduction, in Asymmetric Catalysis on Industrial Scale: Challenges, Approaches and Solutions (eds H.-U. Blaser and E. Schmidt), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527602151.ch12
Editor Information
- 2
Solvias AG, P.O. Box, CH-4002 Basel, Switzerland
- 3
Syngenta Crop Protection AG, CH-4058 Basel, Switzerland
Publication History
- Published Online: 13 JAN 2004
- Published Print: 6 NOV 2003
ISBN Information
Print ISBN: 9783527306312
Online ISBN: 9783527602155
- Summary
- Chapter
Keywords:
- biocatalysis;
- chiral alcohols;
- (R)-1,3-butanediol;
- stereospecific oxidoreduction;
- microorganisms;
- asymmetric reduction;
- racemate;
- Candida parapsilosis IFO 1396;
- cloning;
- gene coding;
- Eschericha coli;
- recombinant cells
Summary
This chapter contains sections titled:
Introduction
Screening of Microorganisms Producing Optically Active 1,3-BDO from 4-Hydroxy-2-butanone (4H2B) by Asymmetric Reduction
Screening of Microorganisms Producing Optically Active 1,3-BDO from the Racemate
Preparation of (R)- and (S)-1,3-BDO by the Same Strain or from the Same Material
Large-Scale Preparation of (R)-1,3-BDO from the Racemate Using Candida parapsilosis IFO 1396
Purification and Characterization of (S)-1,3-Butanediol Dehydrogenase from Candida parapsilosis IFO 1396
Cloning and Expression of a Gene Coding for a Secondary Alcohol Dehydrogenase from Candida parapsilosis IFO 1396 in Eschericha coli
Preparation of Ethyl (R)-4-Chloro-3-hydroxybutanoate (ECHB) by Recombinant E. coli Cells Expressing CpSADH
Conclusion and Outlook