Chapter 10. Protein Engineering

  1. Prof. Dr. Andreas Sebastian Bommarius1,
  2. Dr. Bettina R. Riebel2

Published Online: 28 JAN 2005

DOI: 10.1002/3527602364.ch10

Book Title

Biocatalysis

Biocatalysis

Additional Information

How to Cite

Bommarius, A. S. and Riebel, B. R. (2005) Protein Engineering, in Biocatalysis, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527602364.ch10

Author Information

  1. 1

    School of Chemical and Biomolecular Engineering, Parker H. Petit Biotechnology Institute, Georgia Institute of Technology, 315 Ferst Drive, N. W. Atlanta, GA 30332-0363, USA

  2. 2

    Emory University School of Medicine, Whitehead Research Building, 615 Michael Street, Atlanta, GA 30322, USA

Publication History

  1. Published Online: 28 JAN 2005
  2. Published Print: 28 JAN 2004

ISBN Information

Print ISBN: 9783527303441

Online ISBN: 9783527602360

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Keywords:

  • protein engineering;
  • fusion PCR;
  • Kunkel method;
  • QuikChange kit;
  • combined chain reaction (CCR);
  • glucose isomerase (GI);
  • glycoamylase;
  • thermostability;
  • oxidation stability;
  • subtilisin;
  • lactate dehydrogenase;
  • synthetic peroxidases;
  • oxygenases;
  • enantioselectivity

Summary

  • Introduction: Elements of Protein Engineering

  • Methods of Protein Engineering

    • Fusion PCR

    • Kunkel Method

    • Site-Specific Mutagenesis Using the QuikChange Kit from Stratagene

    • Combined Chain Reaction (CCR)

  • Glucose (Xylose) Isomerase (GI) and Glycoamylase: Enhancement of Thermostability

    • Enhancement of Thermostability in Glucose Isomerase (GI)

    • Resolving the Reaction Mechanism of Glucose Isomerase (GI): Diffusion-Limited Glucose Isomerase?

  • Enhancement of Stability of Proteases against Oxidation and Thermal Deactivation

    • Enhancement of Oxidation Stability of Subtilisin

    • Thermostability of Subtilisin

  • Creating New Enzymes with Protein Engineering

    • Redesign of a Lactate Dehydrogenase

    • Synthetic Peroxidases

  • Dehydrogenases, Changing Cofactor Specificity

  • Oxygenases

  • Change of Enantioselectivity with Site-Specific Mutagenesis

  • Techniques Bridging Different Protein Engineering Techniques

    • Chemically Modified Mutants, a Marriage of Chemical Modification and Protein Engineering

    • Expansion of Substrate Specificity with Protein Engineering and Directed Evolution

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