Chapter 4. Molecular Biology Tools for Biocatalysis

  1. Prof. Dr. Andreas Sebastian Bommarius1,
  2. Dr. Bettina R. Riebel2

Published Online: 28 JAN 2005

DOI: 10.1002/3527602364.ch4

Book Title

Biocatalysis

Biocatalysis

Additional Information

How to Cite

Bommarius, A. S. and Riebel, B. R. (2005) Molecular Biology Tools for Biocatalysis, in Biocatalysis, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527602364.ch4

Author Information

  1. 1

    School of Chemical and Biomolecular Engineering, Parker H. Petit Biotechnology Institute, Georgia Institute of Technology, 315 Ferst Drive, N. W. Atlanta, GA 30332-0363, USA

  2. 2

    Emory University School of Medicine, Whitehead Research Building, 615 Michael Street, Atlanta, GA 30322, USA

Publication History

  1. Published Online: 28 JAN 2005
  2. Published Print: 28 JAN 2004

ISBN Information

Print ISBN: 9783527303441

Online ISBN: 9783527602360

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CHAPTER TOOLS

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Keywords:

  • biocatalysis;
  • DNA;
  • protein;
  • DNA isolation;
  • DNA purification;
  • RNA;
  • gene isolation;
  • gene detection;
  • gene verification;
  • polymerase chain reaction (PCR);
  • DNA-sequencing;
  • cloning techniques;
  • ligation;
  • enzyme function;
  • E. coli;
  • eukaryotic genes;
  • yeasts

Summary

  • Molecular Biology Basics: DNA versus Protein Level

  • DNA Isolation and Purification

    • Quantification of DNA/RNA

  • Gene Isolation, Detection, and Verification

    • Polymerase Chain Reaction

    • Optimization of a PCR Reaction

    • Special PCR Techniques

    • Southern Blotting

    • DNA-Sequencing

  • Cloning Techniques

    • Restriction Mapping

    • Vectors

    • Ligation

  • (Over)expression of an Enzyme Function in a Host

    • Choice of an Expression System

    • Translation and Codon Usage in E. coli

    • Choice of Vector

    • Expression of Eukaryotic Genes in Yeasts

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