Chapter 5. Enzyme Reaction Engineering

  1. Prof. Dr. Andreas Sebastian Bommarius1,
  2. Dr. Bettina R. Riebel2

Published Online: 28 JAN 2005

DOI: 10.1002/3527602364.ch5

Book Title

Biocatalysis

Biocatalysis

Additional Information

How to Cite

Bommarius, A. S. and Riebel, B. R. (2005) Enzyme Reaction Engineering, in Biocatalysis, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527602364.ch5

Author Information

  1. 1

    School of Chemical and Biomolecular Engineering, Parker H. Petit Biotechnology Institute, Georgia Institute of Technology, 315 Ferst Drive, N. W. Atlanta, GA 30332-0363, USA

  2. 2

    Emory University School of Medicine, Whitehead Research Building, 615 Michael Street, Atlanta, GA 30322, USA

Publication History

  1. Published Online: 28 JAN 2005
  2. Published Print: 28 JAN 2004

ISBN Information

Print ISBN: 9783527303441

Online ISBN: 9783527602360

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Keywords:

  • enzyme reaction engineering;
  • kinetic modeling;
  • ideal kinetics;
  • ideal reactors;
  • Michaelis–Menten equation;
  • inhibition;
  • inhibitors;
  • KI–[I]50;
  • enzyme reactors;
  • enzyme reactions;
  • diffusion;
  • film diffusion;
  • pore diffusion;
  • deactivation kinetics;
  • enzyme stability;
  • selectivity;
  • E-value

Summary

  • Kinetic Modeling: Rationale and Purpose

  • The Ideal World: Ideal Kinetics and Ideal Reactors

    • The Classic Case: Michaelis–Menten Equation

    • Design of Ideal Reactors

    • Integrated Michaelis–Menten Equation in Ideal Reactors

  • Enzymes with Unfavorable Binding: Inhibition

    • Types of Inhibitors

    • Integrated Michaelis–Menten Equation for Substrate and Product Inhibition

    • The KI–[I]50 Relationship: Another Useful Application of Mechanism Elucidation

  • Reactor Engineering

    • Configuration of Enzyme Reactors

    • Immobilized Enzyme Reactor (Fixed-Bed Reactor with Plug-Flow)

    • Enzyme Membrane Reactor (Continuous Stirred Tank Reactor, CSTR)

    • Rules for Choice of Reaction Parameters and Reactors

  • Enzyme Reactions with Incomplete Mass Transfer: Influence of Immobilization

    • External Diffusion (Film Diffusion)

    • Internal Diffusion (Pore Diffusion)

    • Methods of Testing for Mass Transfer Limitations

    • Influence of Mass Transfer on the Reaction Parameters

  • Enzymes with Incomplete Stability: Deactivation Kinetics

    • Resting Stability

    • Operational Stability

    • Comparison of Resting and Operational Stability

    • Strategy for the Addition of Fresh Enzyme to Deactiving Enzyme in Continuous Reactors

  • Enzymes with Incomplete Selectivity: E-Value and its Optimization

    • Derivation of the E-Value

    • Optimization of Separation of Racemates by Choice of Degree of Conversion

    • Optimization of Enantiomeric Ratio E by Choice of Temperature

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