Chapter 17. Sequencing of Oligosaccharides and Glycoproteins

  1. Prof. Dr. Chi-Huey Wong
  1. Stuart M. Haslam,
  2. Kay-Hooi Khoo,
  3. Anne Dell

Published Online: 28 JAN 2005

DOI: 10.1002/3527602437.ch17

Book Title

Carbohydrate-Based Drug Discovery

Carbohydrate-Based Drug Discovery

Additional Information

How to Cite

Haslam, S. M., Khoo, K.-H. and Dell, A. (2005) Sequencing of Oligosaccharides and Glycoproteins, in Carbohydrate-Based Drug Discovery (ed C.-H. Wong), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527602437.ch17

Editor Information

  1. Ernest W. Hahn Chair in Chemistry, The Scripps Research Institute, 10550 N. Torrey Pines RoadLa Jolla, CA 92037, USA

Publication History

  1. Published Online: 28 JAN 2005
  2. Published Print: 26 AUG 2003

ISBN Information

Print ISBN: 9783527306329

Online ISBN: 9783527602438

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Keywords:

  • sequencing of oligosaccharides and glycoproteins;
  • mass spectrometry;
  • glycan sequencing;
  • mammalian glycomics

Summary

  • Mass Spectrometry

    • EI-, FAB-, and MALDI-MS

    • ES, NanoES, and LC-MS

    • MS/MS and Mass Analyzers

  • MS-Based Sequencing Strategies

    • Chemical Derivatization

    • MS/MS Fragmentation Patterns

    • Permethylation and Sequence Assignment from Fragment Ions

  • Glycan Sequencing and Structural Determination – A Case Study

    • GC-MS Sugar Analysis

    • Glycan Derivatization

    • FAB-MS of the Deuteroreduced Permethylated HSP Sample

    • ES-MS/MS

    • Linkage Analysis

    • Chemical Hydrolysis

    • Exo-Glycosidase Digestion

  • Mammalian Glycomics

  • Some Special Case Strategies

  • References

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