Chapter 19. Glycosylation Analysis of a Recombinant P-Selectin Antagonist by High-pH Anion-Exchange Chromatography with Pulsed Electrochemical Detection (HPAEC/PED)
- Prof. Dr. Chi-Huey Wong
Published Online: 28 JAN 2005
DOI: 10.1002/3527602437.ch19
Copyright © 2003 Wiley-VCH Verlag GmbH & Co. KGaA
Book Title
Carbohydrate-Based Drug Discovery
Additional Information
How to Cite
Hardy, M. R. and Cornell, R. J. (2005) Glycosylation Analysis of a Recombinant P-Selectin Antagonist by High-pH Anion-Exchange Chromatography with Pulsed Electrochemical Detection (HPAEC/PED), in Carbohydrate-Based Drug Discovery (ed C.-H. Wong), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527602437.ch19
Editor Information
Ernest W. Hahn Chair in Chemistry, The Scripps Research Institute, 10550 N. Torrey Pines RoadLa Jolla, CA 92037, USA
Publication History
- Published Online: 28 JAN 2005
- Published Print: 26 AUG 2003
ISBN Information
Print ISBN: 9783527306329
Online ISBN: 9783527602438
- Summary
- Chapter
Keywords:
- recombinant P-selectin antagonist;
- glycosylation analysis;
- high-pH anion-exchange chromatography with pulsed electrochemical detection (HPAEC/PED);
- biopharmaceuticals;
- biology of P-selectin
Summary
Introduction
Use of HPAEC/PED in the Development of Biopharmaceuticals
Biology of P-Selectin
Structures of PSGL-1 and rPSGL-Ig
HPAEC/PED as an Adjunct to rPSGL-Ig Process Development
Materials and Methods
HPAEC/PED O-Linked Oligosaccharide Profile Analysis
N-Linked Oligosaccharide Profile Analysis
Results and Discussion
HPAEC/PED Oligosaccharide Profile Analysis of a Developmental Batch of rPSGL-Ig
Repeatability of the O-Linked Oligosaccharide Profile Method
O-Glycosylation of rPSGL-Ig Expressed by Different Cell Lines
N-Glycosylation of rPSGL-Ig
Summary
Acknowledgements
References