Chapter 20. Analytical Techniques for the Characterization and Sequencing of Glycosaminoglycans

  1. Prof. Dr. Chi-Huey Wong
  1. Ram Sasisekharan,
  2. Zachary Shriver,
  3. Mallik Sundaram,
  4. Ganesh Venkataraman

Published Online: 28 JAN 2005

DOI: 10.1002/3527602437.ch20

Book Title

Carbohydrate-Based Drug Discovery

Carbohydrate-Based Drug Discovery

Additional Information

How to Cite

Sasisekharan, R., Shriver, Z., Sundaram, M. and Venkataraman, G. (2005) Analytical Techniques for the Characterization and Sequencing of Glycosaminoglycans, in Carbohydrate-Based Drug Discovery (ed C.-H. Wong), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527602437.ch20

Editor Information

  1. Ernest W. Hahn Chair in Chemistry, The Scripps Research Institute, 10550 N. Torrey Pines RoadLa Jolla, CA 92037, USA

Publication History

  1. Published Online: 28 JAN 2005
  2. Published Print: 26 AUG 2003

ISBN Information

Print ISBN: 9783527306329

Online ISBN: 9783527602438

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Keywords:

  • glycosaminoglycans (GAG);
  • characterization;
  • sequencing;
  • depolymerization;
  • detection of GAG oligosaccharides

Summary

  • Introduction to GAG Linear Complex Polysaccharides

  • Depolymerization of Nascent GAG Chains

    • Enzymes that Degrade GAGs

    • Chemical Methods for Degrading GAG Oligosaccharides

  • Detection of GAG Oligosaccharides

    • Δ4,5 Bond Formation and UV Detection

    • Fluorescent Tagging

    • Metabolic Labeling

  • Analytical Tools Used in the Structural Characterization of GAGs

    • High Pressure Liquid Chromatography

      • Amino-Bonded Silica

      • High-Performance Gel Permeation

      • Weak and Strong Anion Exchange

      • Pellicular Anion Exchange

      • IP-RPHPLC

      • Sequencing GAGs I: HPLC Methods

    • Polyacrylamide Gel Electrophoresis

      • Sequencing GAGs II: PAGE Methods

    • Capillary Electrophoresis

    • NMR Spectroscopy

    • Mass Spectrometry

      • Sequencing GAGs III: Mass Spectrometric Methodologies

    • Oligosaccharide Array Technologies

  • Future Directions

  • Acknowledgements

  • References

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