Chapter 19. DNA–Gold-Nanoparticle Conjugates

  1. Prof. Dr. Christof M. Niemeyer2 and
  2. Prof. Dr. Chad A. Mirkin3
  1. C. Shad Thaxton1 and
  2. Prof. Dr. Chad A. Mirkin3

Published Online: 28 JAN 2005

DOI: 10.1002/3527602453.ch19

Nanobiotechnology: Concepts, Applications and Perspectives

Nanobiotechnology: Concepts, Applications and Perspectives

How to Cite

Thaxton, C. S. and Mirkin, C. A. (2004) DNA–Gold-Nanoparticle Conjugates, in Nanobiotechnology: Concepts, Applications and Perspectives (eds C. M. Niemeyer and C. A. Mirkin), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527602453.ch19

Editor Information

  1. 2

    Universität Dortmund, Fachbereich Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto-Hahn-Str. 6, 44227 Dortmund, Germany

  2. 3

    Department of Chemistry & Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA

Author Information

  1. 1

    Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA

  2. 3

    Department of Chemistry & Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA

Publication History

  1. Published Online: 28 JAN 2005
  2. Published Print: 28 JAN 2004

ISBN Information

Print ISBN: 9783527306589

Online ISBN: 9783527602452

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Keywords:

  • DNA-based nanostructures;
  • DNA-gold nanoparticle conjugates;
  • homogeneous DNA detection;
  • biobarcodes;
  • nanoparticle synthesis

Summary

This chapter contains sections titled:

  • Overview

    • Introduction

    • Nanoparticles

    • DNA-functionalized Gold Nanoparticles

    • Nanoparticle Based DNA and RNA Detection Assays

      • Homogeneous DNA Detection

      • Chip-based (Heterogeneous) DNA Detection Assays

    • DNA-Nanoparticle Detection of Proteins: Biobarcodes

    • Conclusion

  • The Essentials: Methods and Protocols

    • Nanoparticle Synthesis

    • DNA-functionalized Au-NP Probe Synthesis

    • Chip Functionalization with DNA Target “Capture” Strands

    • Typical Assay Design

  • Outlook

    • Challenges Ahead

    • Academic and Commercial Applications