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Appendix: Trouble-Shooting: Isoelectric Focusing

  1. Dr. Reiner Westermeier

Published Online: 31 MAR 2005

DOI: 10.1002/3527603468.app1

Electrophoresis in Practice: A Guide to Methods and Applications of DNA and Protein Separations, Fourth Edition

Electrophoresis in Practice: A Guide to Methods and Applications of DNA and Protein Separations, Fourth Edition

How to Cite

Westermeier, R. (2004) Appendix: Trouble-Shooting: Isoelectric Focusing, in Electrophoresis in Practice: A Guide to Methods and Applications of DNA and Protein Separations, Fourth Edition, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603468.app1

Author Information

  1. GE Healthcare Bio-Sciences, Munzinger Str. 9, 79111 Freiburg, Germany

Publication History

  1. Published Online: 31 MAR 2005
  2. Published Print: 25 OCT 2004

ISBN Information

Print ISBN: 9783527311811

Online ISBN: 9783527603466

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Keywords:

  • electrophoresis in practice;
  • trouble-shooting;
  • isoelectric focusing;
  • PAGIEF;
  • carrier ampholytes;
  • agarose IEF;
  • immobilized pH gradients;
  • SDS electrophoresis;
  • vertical PAGE semidry blotting;
  • 2-D electrophoresis;
  • DNA electrophoresis;
  • references

Summary

This appendix contains sections titled:

  • A1 Appendix Trouble-shooting : Isoelectric focusing

    • A1.1 PAGIEF with carrier ampholytes

    • A1.2 Agarose IEF with carrier ampholytes

    • A1.3 Immobilized pH gradients

  • A2 Appendix Trouble-shooting: SDS electrophoresis

  • A3 Appendix Trouble-shooting: Vertical PAGE

  • A4 Appendix Trouble-shooting: Semidry blotting

  • A5 Appendix Trouble-shooting: 2-D electrophoresis

  • A6 Appendix Trouble-shooting: DNA electrophoresis