Chapter 8. Single-Molecule Detection and Manipulation in Nanotechnology and Biology

  1. Dr. Challa S. S. R. Kumar2,
  2. Prof. Dr. Josef Hormes2 and
  3. Prof. Dr. Carola Leuschner3
  1. Christopher L. Kuyper,
  2. Gavin D. M. Jeffries,
  3. Robert M. Lorenz and
  4. Daniel T. Chiu

Published Online: 12 APR 2005

DOI: 10.1002/3527603476.ch8

Nanofabrication Towards Biomedical Applications: Techniques, Tools, Applications, and Impact

Nanofabrication Towards Biomedical Applications: Techniques, Tools, Applications, and Impact

How to Cite

Kuyper, C. L., Jeffries, G. D. M., Lorenz, R. M. and Chiu, D. T. (2005) Single-Molecule Detection and Manipulation in Nanotechnology and Biology, in Nanofabrication Towards Biomedical Applications: Techniques, Tools, Applications, and Impact (eds C. S. S. R. Kumar, J. Hormes and C. Leuschner), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603476.ch8

Editor Information

  1. 2

    Center for Advanced Microstructures and Devices, Louisiana State University, 6980 Jefferson Highway, Baton Rouge, LA 70806, USA

  2. 3

    Reproductive Biotechnology Laboratory, Pennington Biomedical Research Centre, Louisiana State University, 6400 Perkins Road, Baton Rouge, LA 70808, USA

Author Information

  1. University of Washington, Department of Chemistry, P.O. Box 351700, Seattle, WA 98195-1700, USA

Publication History

  1. Published Online: 12 APR 2005
  2. Published Print: 20 JAN 2005

ISBN Information

Print ISBN: 9783527311156

Online ISBN: 9783527603473

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Keywords:

  • nanofabrication;
  • biomedical applications;
  • characterization tools;
  • nanomaterials and nanosystems;
  • single-molecule detection;
  • optical detection;
  • single-molecule manipulations;
  • applications in single-molecule spectroscopy;
  • bright fluorescent species;
  • nanoscale chemistry

Summary

This chapter contains sections titled:

  • Introduction

  • Optical Detection of Single Molecules

    • Detecting Single Molecules with Confocal Fluorescence Microscopy

    • Visualizing Single Molecules with Epifluorescence Detection

    • Total Internal-Reflection Fluorescence (TIRF) Microscopy

    • Single-Molecule Surface-Enhanced Resonance Raman Spectroscopy

  • Single-Molecule Manipulations Using Optical Traps

    • Force Studies Using Single-Beam Gradient Traps

    • Optical Vortex Trapping

    • Optical Arrays

  • Applications in Single-Molecule Spectroscopy

    • Conformational Dynamics of Single DNA Molecules in Solution

    • Probing the Kinetics of Single Enzyme Molecules

    • Single-Molecule DNA Detection, Sorting, and Sequencing

    • Single-Molecule Imaging in Living Cells

  • Single-Molecule Detection with Bright Fluorescent Species

    • Optical Probes

    • Quantum Dots

  • Nanoscale Chemistry with Vesicles and Microdroplets

  • Perspectives