Chapter 3. Columns, Packings and Stationary Phases

  1. Prof. Dr.-Ing. Henner Schmidt-Traub
  1. Prof. Dr. K. K. Unger1,
  2. Dr. Cédric du Fresne von Hohenesche2,
  3. Dr. M. Schulte3

Published Online: 12 APR 2005

DOI: 10.1002/3527603484.ch3

Book Title

Preparative Chromatography: of Fine Chemicals and Pharmaceutical Agents

Preparative Chromatography: of Fine Chemicals and Pharmaceutical Agents

Additional Information

How to Cite

Unger, K. K., du Fresne von Hohenesche, C. and Schulte, M. (2005) Columns, Packings and Stationary Phases, in Preparative Chromatography: of Fine Chemicals and Pharmaceutical Agents (ed H. Schmidt-Traub), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603484.ch3

Editor Information

  1. Universität Dortmund, Fachbereich Bio- und Chemieingenieurwesen, Lehrstuhl für Anlagentechnik, Emil-Figge-Str. 70, 44221 Dortmund, Germany

Author Information

  1. 1

    Am Alten Berg 40, 64342 Seeheim, Germany

  2. 2

    Institut de Science et d'Ingénierie Supramoléculaires, ISIS – BASF Laboratory, 8, allée Gaspard Monge, 67083 Strasbourg, France

  3. 3

    Merck KGaA, Life Science & Analytics R&D, Frankfurter Str. 250, 64293 Darmstadt, Germany

Publication History

  1. Published Online: 12 APR 2005
  2. Published Print: 26 JAN 2005

ISBN Information

Print ISBN: 9783527306435

Online ISBN: 9783527603480

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CHAPTER TOOLS

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Keywords:

  • preparative chromatography;
  • column design;
  • column packings;
  • stationary phases;
  • column testing;
  • column maintenance and regeneration

Summary

This chapter contains sections titled:

  • Column Design

    • Column Hardware and Dimensions

    • Columns with Particles (Particulate Column Beds)

    • Columns with a Continuous Bed (Monolithic Columns)

    • Column Pressure Drop

    • Frit Design

  • Column Packings

    • Survey of Packings and Stationary Phases

    • Generic, Designed and Tailored Adsorbents

      • Generic Adsorbents

      • Tailored Adsorbents

      • Designed Adsorbents

    • Reversed Phase Silicas

      • Silanisation of the Silica Surface

      • Reversed Phase Packings with Polymer Coatings (Types of Polymer Coatings)

      • Physico-chemical Properties of Reversed Phase Silicas

      • Chromatographic Characterization of Reversed Phase Silicas

    • Cross-linked Organic Polymers

      • General Aspects

      • Hydrophobic Polymer Stationary Phases

    • Chiral Stationary Phases

    • Properties of Packings and their Relevance to Chromatographic Performance

      • Chemical and Physical Bulk Properties

  • Column Packing Technology

    • Characterization of the Column Bed Structure

    • Assessment of Column Performance

  • Column Testing

    • Test Systems

    • Hydrodynamic Properties and Column Efficiency

    • Mass Loadability

    • Comparative Rating of Columns

  • Column Maintenance and Regeneration

    • Cleaning in Place (CIP)

    • Conditioning of Silica Surfaces

    • Sanitization in Place (SIP)

    • Column and Adsorbent Storage

  • Guidelines for Choosing Chromatographic Columns and Stationary Phases

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