Chapter 4. Selection of Chromatographic Systems

  1. Prof. Dr.-Ing. Henner Schmidt-Traub
  1. Dipl.-Ing. W. Wewers1,
  2. Dr. J. Dingenen2,
  3. Dr. M. Schulte3,
  4. Prof. Dr. J. Kinkel4

Published Online: 12 APR 2005

DOI: 10.1002/3527603484.ch4

Book Title

Preparative Chromatography: of Fine Chemicals and Pharmaceutical Agents

Preparative Chromatography: of Fine Chemicals and Pharmaceutical Agents

Additional Information

How to Cite

Wewers, W., Dingenen, J., Schulte, M. and Kinkel, J. (2005) Selection of Chromatographic Systems, in Preparative Chromatography: of Fine Chemicals and Pharmaceutical Agents (ed H. Schmidt-Traub), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603484.ch4

Editor Information

  1. Universität Dortmund, Fachbereich Bio- und Chemieingenieurwesen, Lehrstuhl für Anlagentechnik, Emil-Figge-Str. 70, 44221 Dortmund, Germany

Author Information

  1. 1

    University of Dortmund, Department of Biochemical and Chemical Engineering, Chair of Plant and Process Design, 44221 Dortmund, Germany

  2. 2

    Johnson & Johnson, Pharmaceutical Research & Development, Turnhoutseweg 30, 2340 Beerse, Belgium

  3. 3

    Merck KGaA, Life Science & Analytics R&D, Frankfurter Str. 250, 64293 Darmstadt, Germany

  4. 4

    Georg Simon Ohm Fachhochschule Nürnberg, Department of Applied Chemistry, 90489 Nürnberg, Germany

Publication History

  1. Published Online: 12 APR 2005
  2. Published Print: 26 JAN 2005

ISBN Information

Print ISBN: 9783527306435

Online ISBN: 9783527603480

SEARCH

CHAPTER TOOLS

Get PDF (2004K)

Keywords:

  • preparative chromatography;
  • definition of the task;
  • properties of chromatographic systems;
  • criteria for choice of chromatographic systems;
  • criteria for choosing RP systems;
  • criteria for choosing CSP systems

Summary

This chapter contains sections titled:

  • Definition of the Task

  • Properties of Chromatographic Systems

    • Mobile Phases for Liquid Chromatography

      • Stability

      • Safety Concerns

      • Operating Conditions

    • Adsorbent and Phase System

      • Normal Phase System

      • Reversed Phase Chromatography

  • Criteria for Choice of Chromatographic Systems

    • Choice of Phase System Dependent on Solubility

      • Improving Loadability for Poor Solubilities

      • Dependency of Solubility on Sample Purity

      • Generic Gradients for Fast Separations

    • Criteria for Choice of NP Systems

      • Pilot Technique Thin-layer Chromatography

      • Retention in NP Systems

      • Solvent Strength in Liquid–Solid Chromatography

      • Selectivity in NP Systems

      • Mobile Phase Optimization by TLC Following the PRISMA Model

      • Strategy for an Industrial Preparative Chromatography Laboratory

    • Criteria for Choosing RP Systems

      • Retention and Selectivity in RP Systems

      • Gradient Elution for Small amounts of Product on RP Materials

      • Rigorous Optimization for Isocratic Runs

      • Rigorous Optimization for Gradient Runs

      • Practical Recommendations

    • Criteria for Choosing CSP Systems

      • Suitability of Preparative CSP

      • Development of Enantioselectivity

      • Optimization of Separation Conditions

      • Practical Recommendations

    • Conflicts During Optimization of Chromatographic Systems

Get PDF (2004K)