Chapter 11. Whole-Genome Physical Mapping: An Overview on Methods for DNA Fingerprinting

  1. Prof. Dr. Khalid Meksem3,
  2. Prof. Dr. Günter Kahl4
  1. Chengcang Wu1,
  2. Shuku Sun2,
  3. Mi-Kyung Lee2,
  4. Zhanyou Xu2,
  5. Chengwei Ren2,
  6. Teofila S. Santos2,
  7. Hong-Bin Zhang2

Published Online: 28 APR 2005

DOI: 10.1002/3527603514.ch11

Book Title

The Handbook of Plant Genome Mapping: Genetic and Physical Mapping

The Handbook of Plant Genome Mapping: Genetic and Physical Mapping

Additional Information

How to Cite

Wu, C., Sun, S., Lee, M.-K., Xu, Z., Ren, C., Santos, T. S. and Zhang, H.-B. (2005) Whole-Genome Physical Mapping: An Overview on Methods for DNA Fingerprinting, in The Handbook of Plant Genome Mapping: Genetic and Physical Mapping (eds K. Meksem and G. Kahl), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603514.ch11

Editor Information

  1. 3

    Dept. Plant, Soil, Agriculture Southern Illinois University, Carbondale, IL 629, USA

  2. 4

    Plant Molecular Biology Biocentre and GenXPro Johann Wolfgang Goethe University Marie-Curie-Strasse 9 60439 Frankfurt am Main, Germany

Author Information

  1. 1

    Laboratory for Plant Genomics & GENEfinder Genomic Resources, Department of Soil & Crop Sciences, Crop Biotechnology Center, Texas A&M University, College Station, TX 77843-2123, USA

  2. 2

    Laboratory for Plant Genomics & GENEfinder Genomic Resources, Department of Soil & Crop Sciences and Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843-2123, USA

Publication History

  1. Published Online: 28 APR 2005
  2. Published Print: 26 JAN 2005

ISBN Information

Print ISBN: 9783527311163

Online ISBN: 9783527603510

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CHAPTER TOOLS

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Keywords:

  • plant genome mapping;
  • physical mapping;
  • methods for DNA fingerprinting;
  • clone-based hole-genome physical mapping;
  • techniques and methodologies;
  • preparation of DNA;
  • fingerprinting the clone DANN;
  • restriction enzymes;
  • applications of DNA fingerprinting for genome physical mapping

Summary

This chapter contains sections titled:

  • Introduction

    • Inception and Development of DNA Fingerprinting

    • Clone-based Whole-genome Physical Mapping

    • Source DNA Libraries for Whole-genome Physical Mapping

  • Techniques and Methodologies

    • Preparation of DNA from Bacteria-based Large-insert Clones

      • Isolation of DNA from Bacteria-based Large-insert Clones Using the Clone-by-Clone Approach

      • Isolation of DNA from Bacteria-based Large-insert Clones in 96-well Format Using a Manual Approach

      • Isolation of DNA from Bacteria-based Large-insert Clones in 96-well Format Using the AutoGenprep 960 Robotic Workstation

    • Fingerprinting the Clone DNA Using Restriction Enzymes

      • Agarose Gel-based, Restriction Fingerprinting Method

      • Polyacrylamide (Sequencing) Gel-based, Restriction Fingerprinting Method

      • Automatic Sequencing Gel-and Capillary Electrophoresis-based Restriction Fingerprinting Methods

    • Applications of DNA Fingerprinting for Genome Physical Mapping

      • Whole-genome Physical Maps Generated from Large-insert Bacterial Clones by Fingerprint Analysis

      • Comparison of Different Fingerprinting Methods

  • Discussion

  • Acknowledgements

  • References

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