Chapter 13. Reduced Representation Strategies and Their Application to Plant Genomes

  1. Prof. Dr. Khalid Meksem2,
  2. Prof. Dr. Günter Kahl3
  1. Daniel G. Peterson

Published Online: 28 APR 2005

DOI: 10.1002/3527603514.ch13

Book Title

The Handbook of Plant Genome Mapping: Genetic and Physical Mapping

The Handbook of Plant Genome Mapping: Genetic and Physical Mapping

Additional Information

How to Cite

Peterson, D. G. (2005) Reduced Representation Strategies and Their Application to Plant Genomes, in The Handbook of Plant Genome Mapping: Genetic and Physical Mapping (eds K. Meksem and G. Kahl), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603514.ch13

Editor Information

  1. 2

    Dept. Plant, Soil, Agriculture Southern Illinois University, Carbondale, IL 629, USA

  2. 3

    Plant Molecular Biology Biocentre and GenXPro Johann Wolfgang Goethe University Marie-Curie-Strasse 9 60439 Frankfurt am Main, Germany

Author Information

  1. Mississippi Genome Exploration Laboratory (MGEL), Dept. of Plant and Soil Sciences, 117 Dorman Hall, Box 9555, Mississippi State University, Mississippi State, MS 39762, USA

Publication History

  1. Published Online: 28 APR 2005
  2. Published Print: 26 JAN 2005

ISBN Information

Print ISBN: 9783527311163

Online ISBN: 9783527603510

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CHAPTER TOOLS

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Keywords:

  • plant genome mapping;
  • physical mapping;
  • reduced representation techniques;
  • EST sequencing;
  • methylation filtration;
  • repeat sequence enrichment;
  • sequencing gene space;
  • polymorphism discovery

Summary

This chapter contains sections titled:

  • Introduction

  • Reduced Representation Techniques

    • EST Sequencing

    • Methylation Filtration

    • Cot-based Cloning and Sequencing

    • De Novo Polymorphism Discovery

      • Reduced Representation Shotgun (RRS) Sequencing

      • DOP-PCR

      • SSR Capture

  • Other Reduced Representation Techniques

  • Discussion

    • Repeat Sequence Enrichment

    • Sequencing Gene space

      • EST Sequencing

      • Methylation Filtration

      • Cot-based Cloning and Sequencing

      • Integration of Reduced Representation Strategies

    • Polymorphism Discovery

      • RRS Sequencing and DOP-PCR

      • Microsatellite Isolation

  • Conclusions

  • Acknowledgments

  • References

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