Chapter 14. Large-Scale DNA Sequencing
- Prof. Dr. Khalid Meksem2,
- Prof. Dr. Günter Kahl3
Published Online: 28 APR 2005
DOI: 10.1002/3527603514.ch14
Copyright © 2005 Wiley-VCH Verlag GmbH & Co. KGaA
Book Title
The Handbook of Plant Genome Mapping: Genetic and Physical Mapping
Additional Information
How to Cite
Town, C. D. (2005) Large-Scale DNA Sequencing, in The Handbook of Plant Genome Mapping: Genetic and Physical Mapping (eds K. Meksem and G. Kahl), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603514.ch14
Editor Information
- 2
Dept. Plant, Soil, Agriculture Southern Illinois University, Carbondale, IL 629, USA
- 3
Plant Molecular Biology Biocentre and GenXPro Johann Wolfgang Goethe University Marie-Curie-Strasse 9 60439 Frankfurt am Main, Germany
Publication History
- Published Online: 28 APR 2005
- Published Print: 26 JAN 2005
ISBN Information
Print ISBN: 9783527311163
Online ISBN: 9783527603510
- Summary
- Chapter
Keywords:
- plant genome mapping;
- physical mapping;
- large-scale DNA sequencing;
- EST sequencing;
- chromosome-based sequencing;
- whole-genome shotgun sequencing;
- selective genome sequencing;
- differential cloning strategies;
- low-coverage sequencing;
- BAC end sequencing
Summary
This chapter contains sections titled:
Introduction
Motivation for Sequencing and Choice of Strategy
EST Sequencing
Large-scale Sequencing BAC-based Projects
Chromosome-based Sequencing
Whole-genome Shotgun Sequencing
Selective Genome Sequencing by Differential Cloning Strategies
Low-coverage Sequencing
BAC End Sequencing
The Sequencing Process Itself
Library Production
Template Production
Sequencing Reactions and Analysis
Sequencing Capacity and Costs
Post-sequencing Data Processing
Quality Trimming
Sequence Assembly
Scaffolding
Sequence Editing and Gap Closure
Conclusions
Acknowledgments
References