Chapter 7. Chromosome Flow Sorting and Physical Mapping

  1. Prof. Dr. Khalid Meksem3,
  2. Prof. Dr. Günter Kahl4
  1. Jaroslav Doležel1,
  2. Marie Kubaláková1,
  3. Jan Bartoš1,
  4. Jiří Macas2

Published Online: 28 APR 2005

DOI: 10.1002/3527603514.ch7

Book Title

The Handbook of Plant Genome Mapping: Genetic and Physical Mapping

The Handbook of Plant Genome Mapping: Genetic and Physical Mapping

Additional Information

How to Cite

Doležel, J., Kubaláková, M., Bartoš, J. and Macas, J. (2005) Chromosome Flow Sorting and Physical Mapping, in The Handbook of Plant Genome Mapping: Genetic and Physical Mapping (eds K. Meksem and G. Kahl), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603514.ch7

Editor Information

  1. 3

    Dept. Plant, Soil, Agriculture Southern Illinois University, Carbondale, IL 629, USA

  2. 4

    Plant Molecular Biology Biocentre and GenXPro Johann Wolfgang Goethe University Marie-Curie-Strasse 9 60439 Frankfurt am Main, Germany

Author Information

  1. 1

    Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovska 6, 77200 Olomouc, Czech Republic

  2. 2

    Laboratory of Molecular Cytogenetics, Institute of Plant Molecular Biology, Branisovska 31, 37005 České Budějovice, Czech Republic

Publication History

  1. Published Online: 28 APR 2005
  2. Published Print: 26 JAN 2005

ISBN Information

Print ISBN: 9783527311163

Online ISBN: 9783527603510

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CHAPTER TOOLS

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Keywords:

  • plant genome mapping;
  • physical mapping;
  • chromosome flow sorting;
  • flow cytogenetics for plants;
  • flow karyotyping;
  • cell cycle synchronization;
  • metaphase accumulation;
  • chromosome isolation;
  • chromosome analysis

Summary

This chapter contains sections titled:

  • Introduction

  • Development of Flow Cytogenetics for Plants

    • The Uses of Flow Karyotyping

    • Applications of Flow-sorted Chromosomes

  • Methodologies and Techniques

    • Cell Cycle Synchronization and Metaphase Accumulation

    • Chromosome Isolation

    • Chromosome Analysis

      • Detection of Structural Chromosome Changes

      • Quantitative Detection of Numerical Chromosome Changes

      • Chromosome Sorting

      • Cytogenetic Mapping on Flow-sorted Chromosomes

      • Physical Mapping Using PCR

      • Chromosome and Chromosome Arm–specific DNA Libraries

      • Targeted Isolation of Molecular Markers

  • Discussion

  • Acknowledgments

  • References

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