Chapter 8. Genomic DNA Libraries and Physical Mapping

  1. Prof. Dr. Khalid Meksem4,
  2. Prof. Dr. Günter Kahl5
  1. Chengwei Ren1,
  2. Zhanyou Xu1,
  3. Shuku Sun1,
  4. Mi-Kyung Lee1,
  5. Chengcang Wu2,
  6. Chantel Scheuring3,
  7. Hong-Bin Zhang1

Published Online: 28 APR 2005

DOI: 10.1002/3527603514.ch8

Book Title

The Handbook of Plant Genome Mapping: Genetic and Physical Mapping

The Handbook of Plant Genome Mapping: Genetic and Physical Mapping

Additional Information

How to Cite

Ren, C., Xu, Z., Sun, S., Lee, M.-K., Wu, C., Scheuring, C. and Zhang, H.-B. (2005) Genomic DNA Libraries and Physical Mapping, in The Handbook of Plant Genome Mapping: Genetic and Physical Mapping (eds K. Meksem and G. Kahl), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603514.ch8

Editor Information

  1. 4

    Dept. Plant, Soil, Agriculture Southern Illinois University, Carbondale, IL 629, USA

  2. 5

    Plant Molecular Biology Biocentre and GenXPro Johann Wolfgang Goethe University Marie-Curie-Strasse 9 60439 Frankfurt am Main, Germany

Author Information

  1. 1

    Laboratory for Plant Genomics & GENEfinder Genomic Resources, Department of Soil & Crop Sciences and Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843-2123, USA

  2. 2

    Laboratory for Plant Genomics & GENEfinder Genomic Resources, Department of Soil & Crop Sciences, Crop Biotechnology Center, Texas A&M University, College Station, TX 77843-2123, USA

  3. 3

    Department of Soil and Crop Sciences and Institute for Plant Genomics and Biotechnology, 2123 TAMU, Texas A&M University, College Station, TX 77843-2123, USA

Publication History

  1. Published Online: 28 APR 2005
  2. Published Print: 26 JAN 2005

ISBN Information

Print ISBN: 9783527311163

Online ISBN: 9783527603510

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CHAPTER TOOLS

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Keywords:

  • plant genome mapping;
  • genomic DNA libraries;
  • physical mapping;
  • bacteria-based large-insert DNA clones;
  • bacteria-based large-insert genomic DNA libraries;
  • applications

Summary

This chapter contains sections titled:

  • Introduction

  • Methodologies and Techniques

    • Bacteria-based Large-insert DNA Clones

      • BAC

      • PAC

      • BIBACs

      • PBC

      • TAC

    • Genomic DNA Library Quality and Genome Physical Mapping

      • Insert Sizes

      • Clone Genome Coverage

      • Representation for Genome

      • The Importance of Binary Vectors in Plant Physical Mapping

    • Construction of Bacteria-based Large-insert Genomic DNA Libraries

      • Megabase-size Nuclear DNA Preparation

      • Vector Preparation

      • Library Construction

    • Applications of Bacteria-based Large-insert Genomic DNA Libraries in Genome Physical Mapping

      • Physical Mapping by Fingerprint Analysis

      • Physical Mapping by Fluorescent in situ Hybridization

      • Physical Mapping by Optical Mapping

      • Physical Mapping by Iterative Hybridization

      • Physical Mapping by Other Techniques

  • Discussion

  • Acknowledgments

  • References

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