Chapter 4. Experimental Techniques in a Nutshell

  1. Dr. E. Klipp,
  2. Dr. R. Herwig,
  3. Dr. A. Kowald,
  4. C. Wierling,
  5. Prof. Dr. H. Lehrach

Published Online: 11 MAY 2005

DOI: 10.1002/3527603603.ch4

Book Title

Systems Biology in Practice: Concepts, Implementation and Application

Systems Biology in Practice: Concepts, Implementation and Application

Additional Information

How to Cite

Klipp, E., Herwig, R., Kowald, A., Wierling, C. and Lehrach, H. (2005) Experimental Techniques in a Nutshell, in Systems Biology in Practice: Concepts, Implementation and Application, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603603.ch4

Author Information

  1. MPI für Molekulare Genetik, Ihnestraße 73, 14195 Berlin, Germany

Publication History

  1. Published Online: 11 MAY 2005
  2. Published Print: 17 FEB 2005

ISBN Information

Print ISBN: 9783527310784

Online ISBN: 9783527603602

SEARCH

CHAPTER TOOLS

Get PDF (394K)

Keywords:

  • experimental techniques;
  • restriction enzymes;
  • gel electrophoresis;
  • cloning vectors;
  • DNA libraries;
  • hybridization;
  • blotting techniques;
  • PCR;
  • DNA and protein chips;
  • mass spectrometry

Summary

This chapter contains sections titled:

  • Elementary Techniques

    • Restriction Enzymes and Gel Electrophoresis

    • Cloning Vectors and DNA Libraries

    • 1D and 2D Protein Gels

    • Hybridization and Blotting Techniques

      • Southern Blotting

      • Northern Blotting

      • Western Blotting

      • In situ Hybridization

    • Further Protein Separation Techniques

      • Centrifugation

      • Column Chromatography

  • Advanced Techniques

    • PCR

    • DNA and Protein Chips

      • DNA Chips

      • Protein Chips

    • Yeast Two-hybrid System

    • Mass Spectrometry

    • Transgenic Animals

    • RNA Interference

Get PDF (394K)