Chapter 11. Mammalian Cells
- Prof. Dr. Gerd Gellissen2,3
Published Online: 19 MAY 2005
DOI: 10.1002/3527603670.ch11
Copyright © 2005 Wiley-VCH Verlag GmbH & Co. KGaA
Book Title
Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems
Additional Information
How to Cite
Sandig, V., Rose, T., Winkler, K. and Brecht, R. (2005) Mammalian Cells, in Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems (ed G. Gellissen), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603670.ch11
Editor Information
- 2
MedArtis Pharmaceuticals GmbH, Pauwelsstr. 19, 52047 Aachen, Germany
- 3
Ringstrasse 30, 42489 Wülfrath, Germany
Publication History
- Published Online: 19 MAY 2005
- Published Print: 4 NOV 2004
ISBN Information
Print ISBN: 9783527310364
Online ISBN: 9783527603671
- Summary
- Chapter
Keywords:
- production of recombinant proteins;
- novel microbial and eucaryotic expression systems;
- mammalian cells;
- heterologous gene expression;
- mammalian cell lines for protein production;
- mammalian expression systems;
- mammalian cell-based fermentation processes
Summary
This chapter contains sections titled:
Why Use Mammalian Cells for Heterologous Gene Expression?
Mammalian Cell Lines for Protein Production
Mammalian Expression Systems
Design of the Basic Expression Unit
Transient Expression and Episomal Vectors: Alternatives to Stable Integration
“Stable” Integration into the Host Genome
Selection Strategies for Mammalian Cells
Auxotrophic Selection Markers and Gene Amplification
The Integration Locus: a Major Determinant of Expression Level
Mammalian Cell-based Fermentation Processes
Batch and Fed-batch Fermentation
Continuous Perfusion Fermentation
Continuous Production with Hollow-fiber Bioreactors
Conclusions
Acknowledgments
Appendix
References