Chapter 12. Plant Cells

  1. Prof. Dr. Gerd Gellissen4,5
  1. Rainer Fischer1,
  2. Richard M Twyman2,
  3. Jürgen Drossard1,
  4. Stephan Hellwig3,4,
  5. Stefan Schillberg3

Published Online: 19 MAY 2005

DOI: 10.1002/3527603670.ch12

Book Title

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

Additional Information

How to Cite

Fischer, R., Twyman, R. M., Drossard, J., Hellwig, S. and Schillberg, S. (2005) Plant Cells, in Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems (ed G. Gellissen), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603670.ch12

Editor Information

  1. 4

    MedArtis Pharmaceuticals GmbH, Pauwelsstr. 19, 52047 Aachen, Germany

  2. 5

    Ringstrasse 30, 42489 Wülfrath, Germany

Author Information

  1. 1

    Institute of Molecular Biotechnology, RWTH Aachen University, Worringer Weg 1, 52056 Aachen, Germany

  2. 2

    Department of Biological Sciences, University of York, Heslington, York, YO10 5DD, UK, England

  3. 3

    Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Worringerweg 1, 52074 Aachen, Germany

  4. 4

    MedArtis Pharmaceuticals GmbH, Pauwelsstr. 19, 52047 Aachen, Germany

Publication History

  1. Published Online: 19 MAY 2005
  2. Published Print: 4 NOV 2004

ISBN Information

Print ISBN: 9783527310364

Online ISBN: 9783527603671

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Keywords:

  • production of recombinant proteins;
  • novel microbial and eucaryotic expression systems;
  • plant cells;
  • general biology;
  • description of the expression platform;
  • examples of recombinant proteins produced;
  • plant cell suspension cultures;
  • methodology;
  • model tobacco cell suspension lines;
  • produce recombinant antibodies

Summary

This chapter contains sections titled:

  • General Biology of Plant Cells

    • Advantages of Plant Cells for the Production of Recombinant Proteins

    • N-Glycan Synthesis in Plants

  • Description of the Expression Platform

    • Culture Systems and Expression Hosts

    • Derivation of Suspension Cells

    • Optimizing Protein Accumulation and Recovery

    • Expression Construct Design

    • Foreign Protein Stability

    • Medium Additives that Enhance Protein Accumulation

      • Simple Inorganic Compounds

      • Amino Acids

      • Dimethylsulfoxide

      • Organic Polymers

      • Proteins

    • Other Properties of the Culture Medium

    • Culture and Harvest Processes

  • Examples of Recombinant Proteins Produced in Plant Cell Suspension Cultures

  • Methodology Using Model Tobacco Cell Suspension Lines to Produce Recombinant Antibodies

  • Conclusions

  • Appendix

  • References

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