Chapter 13. Wide-Range Integrative Expression Vectors for Fungi, Based on Ribosomal DNA Elements

  1. Prof. Dr. Gerd Gellissen3,4
  1. Jens Klabunde1,
  2. Gotthard Kunze2,
  3. Prof. Dr. Gerd Gellissen3,4,
  4. Cornelis P. Hollenberg1

Published Online: 19 MAY 2005

DOI: 10.1002/3527603670.ch13

Book Title

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

Additional Information

How to Cite

Klabunde, J., Kunze, G., Gellissen, G. and Hollenberg, C. P. (2005) Wide-Range Integrative Expression Vectors for Fungi, Based on Ribosomal DNA Elements, in Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems (ed G. Gellissen), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603670.ch13

Editor Information

  1. 3

    MedArtis Pharmaceuticals GmbH, Pauwelsstr. 19, 52047 Aachen, Germany

  2. 4

    Ringstrasse 30, 42489 Wülfrath, Germany

Author Information

  1. 1

    Institut für Mikrobiologie, Heinrich-Heine-Universität, Universitätsstr. 1, 40225 Düsseldorf, Germany

  2. 2

    IPK Gatersleben, Corrensstr. 3, 06466 Gatersleben, Germany

  3. 3

    MedArtis Pharmaceuticals GmbH, Pauwelsstr. 19, 52047 Aachen, Germany

  4. 4

    Ringstrasse 30, 42489 Wülfrath, Germany

Publication History

  1. Published Online: 19 MAY 2005
  2. Published Print: 4 NOV 2004

ISBN Information

Print ISBN: 9783527310364

Online ISBN: 9783527603671

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Keywords:

  • production of recombinant proteins;
  • novel microbial and eucaryotic expression systems;
  • wide-range integrative expression vectors for fungi;
  • based on ribosomal DNA elements;
  • structure of the ribosomal DNA;
  • utility of ribosomal DNA as an integration target;
  • transformation based on rDNA integration;
  • rDNA integration;
  • a tool for targeting multiple expression cassettes

Summary

This chapter contains sections titled:

  • Why is a Wide-range Expression Vector Needed?

  • Which Elements are Essential for a Wide-range Expression Vector?

  • Structure of the Ribosomal DNA and its Utility as an Integration Target

    • Organization of the rDNA in Yeast

    • Sequence Characteristics of rDNA

  • Transformation Based on rDNA Integration

  • rDNA Integration as a Tool for Targeting Multiple Expression Cassettes

    • Co-integration of Reporter Plasmids in A. adeninivorans

    • Approaches to the Production of Pharmaceuticals by Co-integration of Different Genes

  • Conclusions and Perspectives

  • Acknowledgments

  • References

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