Chapter 15. Recombinant Hepatitis B Vaccines: Disease Characterization and Vaccine Production

  1. Prof. Dr. Gerd Gellissen4,5
  1. Pascale Brocke1,
  2. Stephan Schaefer2,
  3. Karl Melber1,
  4. Volker Jenzelewski1,
  5. Frank Müller1,
  6. Ulrike Dahlems1,
  7. Oliver Bartelsen1,
  8. Kyung-Nam Park3,
  9. Zbigniew A. Janowicz1,
  10. Prof. Dr. Gerd Gellissen4,5

Published Online: 19 MAY 2005

DOI: 10.1002/3527603670.ch15

Book Title

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

Additional Information

How to Cite

Brocke, P., Schaefer, S., Melber, K., Jenzelewski, V., Müller, F., Dahlems, U., Bartelsen, O., Park, K.-N., Janowicz, Z. A. and Gellissen, G. (2005) Recombinant Hepatitis B Vaccines: Disease Characterization and Vaccine Production, in Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems (ed G. Gellissen), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603670.ch15

Editor Information

  1. 4

    MedArtis Pharmaceuticals GmbH, Pauwelsstr. 19, 52047 Aachen, Germany

  2. 5

    Ringstrasse 30, 42489 Wülfrath, Germany

Author Information

  1. 1

    Rhein Biotech GmbH, Eichsfelder Str. 11, 40595 Düsseldorf, Germany

  2. 2

    University Rostock, Department of Medical Microbiology and Virology, Schillingallee 70, 18055 Rostock, Germany

  3. 3

    227– 3, Kuga-li, Giheung-Eup, Yongin-Shi, Kyunggi-do, Korea

  4. 4

    MedArtis Pharmaceuticals GmbH, Pauwelsstr. 19, 52047 Aachen, Germany

  5. 5

    Ringstrasse 30, 42489 Wülfrath, Germany

Publication History

  1. Published Online: 19 MAY 2005
  2. Published Print: 4 NOV 2004

ISBN Information

Print ISBN: 9783527310364

Online ISBN: 9783527603671

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Keywords:

  • production of recombinant proteins;
  • novel microbial and eucaryotic expression systems;
  • recombinant Hepatitis B vaccines;
  • disease characterization;
  • vaccine production;
  • virus characteristics;
  • the Hepadnaviruses;
  • recombinant vaccine production;
  • HepavaxGene®;
  • alternative vaccine strategies

Summary

This chapter contains sections titled:

  • Introduction

  • Virus and Disease Characteristics

    • The Hepadnaviruses

    • Subtypes of HBV

    • Pathogenesis

    • Immune Response

    • Prophylactic HBV Vaccines

  • Recombinant Vaccine Production

    • Yeasts as Production Organisms

    • Construction of a H. polymorpha Strain Expressing the Hepatitis B S-antigen

      • Expression Cassette and Vector Construction

      • Transformation of H. polymorpha

      • Strain Characterization

    • H. polymorpha-derived HBsAg Production Process

      • Fermentation (Upstream Process)

      • Purification (Downstream Processing)

  • HepavaxGene®

    • Preclinical Studies

    • Clinical Studies

    • Second-generation Prophylactic Vaccine@ SUPERVAX

  • Hepatitis B Vaccines: Past, Present, and Future

    • Use and Success of Prophylactic Hepatitis B Vaccination

    • Current Shortcomings of Hepatitis B Vaccines

      • Non-responders

      • Incomplete Vaccination

      • Escape Variants

    • Alternative Vaccine Strategies

      • Oral Administration of Plant-derived, Edible Vaccines

      • Oral Administration of Live Bacterial Vectors

      • Live Viral Vectors

      • DNA Vaccines

      • Single-dose Vaccines

      • Mammalian Cell-derived, PreS1/preS2-containing Vaccines

      • Novel Adjuvants

    • Treatment of Chronic Hepatitis

    • Combination Vaccines

  • Conclusions

  • References

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