Chapter 2. Escherichia coli
- Prof. Dr. Gerd Gellissen2,3
Published Online: 19 MAY 2005
DOI: 10.1002/3527603670.ch2
Copyright © 2005 Wiley-VCH Verlag GmbH & Co. KGaA
Book Title
Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems
Additional Information
How to Cite
Altenbuchner, J. and Mattes, R. (2005) Escherichia coli, in Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems (ed G. Gellissen), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603670.ch2
Editor Information
- 2
MedArtis Pharmaceuticals GmbH, Pauwelsstr. 19, 52047 Aachen, Germany
- 3
Ringstrasse 30, 42489 Wülfrath, Germany
Publication History
- Published Online: 19 MAY 2005
- Published Print: 4 NOV 2004
ISBN Information
Print ISBN: 9783527310364
Online ISBN: 9783527603671
- Summary
- Chapter
Keywords:
- production of recombinant proteins;
- novel microbial and eucaryotic expression systems;
- Escherichia coli;
- strains;
- genome;
- cultivation;
- expression vectors;
- regulation of gene expression;
- transcription and translation;
- protein production;
- examples of products and processes
Summary
This chapter contains sections titled:
Introduction
Strains, Genome, and Cultivation
Expression Vectors
Replication of pMB1-derived Vectors
Plasmid Partitioning
Genome Engineering
E. coli Promoters
Regulation of Gene Expression
Negative Control
Positive Control
L-Arabinose Operon
L-Rhamnose Operon
Transcription and Translation
Translation Initiation
Codon Usage
Translation Termination
Transcription Termination and mRNA Stability
Protein Production
Inclusion Body Formation
Chaperones as Facilitators of Folding
Fusion Protein Technology
Methionine Processing
Secretion into the Periplasm
Disulfide Bond Formation and Folding
Twin Arginine Translocation (TAT) of Folded Proteins
Disulfide Bond Formation in the Cytoplasm
Cell Surface Display and Secretion across the Outer Membrane
Examples of Products and Processes
Conclusions and Future Perspectives
Appendix
References