Chapter 3. Pseudomonas fluorescens

  1. Prof. Dr. Gerd Gellissen2,3
  1. Lawrence C. Chew,
  2. Tom M. Ramseier,
  3. Diane M. Retallack,
  4. Jane C. Schneider,
  5. Charles H. Squires,
  6. Henry W. Talbot

Published Online: 19 MAY 2005

DOI: 10.1002/3527603670.ch3

Book Title

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

Additional Information

How to Cite

Chew, L. C., Ramseier, T. M., Retallack, D. M., Schneider, J. C., Squires, C. H. and Talbot, H. W. (2005) Pseudomonas fluorescens, in Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems (ed G. Gellissen), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603670.ch3

Editor Information

  1. 2

    MedArtis Pharmaceuticals GmbH, Pauwelsstr. 19, 52047 Aachen, Germany

  2. 3

    Ringstrasse 30, 42489 Wülfrath, Germany

Author Information

  1. The Dow Chemical Company, Biotechnology, Research and Development, 5501 Oberlin Dr., San Diego, CA 92121, USA

Publication History

  1. Published Online: 19 MAY 2005
  2. Published Print: 4 NOV 2004

ISBN Information

Print ISBN: 9783527310364

Online ISBN: 9783527603671

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CHAPTER TOOLS

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Keywords:

  • production of recombinant proteins;
  • novel microbial and eucaryotic expression systems;
  • Pseudomonas fluorescens;
  • biology;
  • history and taxonomy;
  • strain biovar I MB101;
  • cultivation;
  • genomics and functional genomics of P. fluorescens Strain MB101;
  • core expression platform for heterologous proteins;
  • production of heterologous proteins in P. fluorescens

Summary

This chapter contains sections titled:

  • Introduction

  • Biology of Pseudomonas fluorescens

  • History and Taxonomy of Pseudomonas fluorescens Strain Biovar I MB101

  • Cultivation

  • Genomics and Functional Genomics of P. fluorescens Strain MB101

  • Core Expression Platform for Heterologous Proteins

    • Antibiotic-free Plasmids using pyrF and proC

    • Gene Deletion Strategy and Re-usable Markers

    • Periplasmic Secretion and Use of Transposomes

    • Alternative Expression Systems: Anthranilate and Benzoate-inducible Promoters

  • Production of Heterologous Proteins in P. fluorescens

    • Pharmaceutical Proteins

    • Industrial Enzymes

    • Agricultural Proteins

  • Conclusions

  • Appendix

  • References

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