Chapter 6. Hansenula polymorpha

  1. Prof. Dr. Gerd Gellissen2,3
  1. Hyun Ah Kang1,
  2. Prof. Dr. Gerd Gellissen2,3

Published Online: 19 MAY 2005

DOI: 10.1002/3527603670.ch6

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

How to Cite

Kang, H. A. and Gellissen, G. (2005) Hansenula polymorpha, in Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems (ed G. Gellissen), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603670.ch6

Editor Information

  1. 2

    MedArtis Pharmaceuticals GmbH, Pauwelsstr. 19, 52047 Aachen, Germany

  2. 3

    Ringstrasse 30, 42489 Wülfrath, Germany

Author Information

  1. 1

    Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Eoen-dong Yusong-gu, Daejeon 305– 333, Korea

  2. 2

    MedArtis Pharmaceuticals GmbH, Pauwelsstr. 19, 52047 Aachen, Germany

  3. 3

    Ringstrasse 30, 42489 Wülfrath, Germany

Publication History

  1. Published Online: 19 MAY 2005
  2. Published Print: 4 NOV 2004

ISBN Information

Print ISBN: 9783527310364

Online ISBN: 9783527603671

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Keywords:

  • production of recombinant proteins;
  • novel microbial and eucaryotic expression systems;
  • Hansenula polymorpha;
  • history;
  • phylogenetic position;
  • basic genetics and biochemistry of H.polymorpha;
  • characteristics of the H. polymorpha genome;
  • N-linked glycosylation in H. polymorpha;
  • H. polymorpha-based expression platform;
  • product and process examples

Summary

This chapter contains sections titled:

  • History, Phylogenetic Position, Basic Genetics and Biochemistry of H.polymorpha

  • Characteristics of the H. polymorpha Genome

  • N-linked glycosylation in H. polymorpha

  • The H. polymorpha-based Expression Platform

    • Transformation

    • Strains

    • Plasmids and Available Elements

  • Product and Process Examples

  • Future Directions and Conclusion

    • Limitations of the H. polymorpha-based Expression Platform

    • Impact of Functional Genomics on Development of the H. polymorpha RB11-based Expression Platform

  • Appendix

  • References