Chapter 7. Pichia pastoris

  1. Prof. Dr. Gerd Gellissen3,4
  1. Christine Ilgen1,
  2. Joan Lin-Cereghino2,
  3. James M. Cregg Professor and Director of Research1

Published Online: 19 MAY 2005

DOI: 10.1002/3527603670.ch7

Book Title

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

Additional Information

How to Cite

Ilgen, C., Lin-Cereghino, J. and Cregg, J. M. (2005) Pichia pastoris, in Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems (ed G. Gellissen), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603670.ch7

Editor Information

  1. 3

    MedArtis Pharmaceuticals GmbH, Pauwelsstr. 19, 52047 Aachen, Germany

  2. 4

    Ringstrasse 30, 42489 Wülfrath, Germany

Author Information

  1. 1

    Keck Graduate Institute of Applied Sciences, 535 Watson Drive, Claremont, CA 91711, USA

  2. 2

    Department of Biological Sciences, University of the Pacific Stockton, CA 95211, USA

Publication History

  1. Published Online: 19 MAY 2005
  2. Published Print: 4 NOV 2004

ISBN Information

Print ISBN: 9783527310364

Online ISBN: 9783527603671

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CHAPTER TOOLS

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Keywords:

  • production of recombinant proteins;
  • novel microbial and eucaryotic expression systems;
  • Pichia pastoris;
  • construction of expression strains;
  • post-translational modification of secreted proteins

Summary

This chapter contains sections titled:

  • Introduction

  • Construction of Expression Strains

    • Expression Vector Components

    • Alternative Promoters

    • Selectable Markers

    • Host Strains

      • Methanol Utilization Phenotype

      • Protease-deficient Host Strains

    • Construction of Expression Strains

    • Multicopy Strains

    • Growth in Fermentor Cultures

  • Post-translational Modification of Secreted Proteins

    • Secretion Signals

    • O-linked Glycosylation

    • N-linked Glycosylation

    • “Humanization” of N-linked Carbohydrate

  • Conclusions

  • Acknowledgments

  • Appendix

  • References

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