Chapter 8. Yarrowia lipolytica

  1. Prof. Dr. Gerd Gellissen2,3
  1. Catherine Madzak,
  2. Jean-Marc Nicaud,
  3. Claude Gaillardin

Published Online: 19 MAY 2005

DOI: 10.1002/3527603670.ch8

Book Title

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

Additional Information

How to Cite

Madzak, C., Nicaud, J.-M. and Gaillardin, C. (2005) Yarrowia lipolytica, in Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems (ed G. Gellissen), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603670.ch8

Editor Information

  1. 2

    MedArtis Pharmaceuticals GmbH, Pauwelsstr. 19, 52047 Aachen, Germany

  2. 3

    Ringstrasse 30, 42489 Wülfrath, Germany

Author Information

  1. Microbiologie et Génétique Moleculaire, UMR1238 INAPG-INRA-CNRS, Institut National Agronomique, Paris-Grignon, 78850 Thiverval Grignon, France

Publication History

  1. Published Online: 19 MAY 2005
  2. Published Print: 4 NOV 2004

ISBN Information

Print ISBN: 9783527310364

Online ISBN: 9783527603671

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CHAPTER TOOLS

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Keywords:

  • production of recombinant proteins;
  • novel microbial and eucaryotic expression systems;
  • Yarrowia lipolytica;
  • history;
  • phylogenetic position;
  • basic genetics;
  • biochemistry;
  • characteristics of the Y. lipolytica Genome;
  • description of the expression platform;
  • examples;
  • transformation methods

Summary

This chapter contains sections titled:

  • History, Phylogenetic Position, Basic Genetics, and Biochemistry

    • Main Characteristics

    • Historical Perspective on the Development of Studies

    • Secretion of Proteins

    • Production of Heterologous Proteins and Glycosylation

  • Characteristics of the Y. lipolytica Genome

  • Description of the Expression Platform

    • Host Strains

    • Selection Markers and Expression Signals

      • Selection Markers

      • The Expression Cassette

        • Promoters

        • Secretion Targeting Signals

    • Shuttle Vectors for Heterologous Protein Expression

      • Replicative Vectors

      • Integrative Vectors

  • Examples

  • Transformation Methods

    • Materials

    • Preparation of Competent Cells

    • Transformation

  • Conclusions and Future Trends

  • Acknowledgments

  • Appendix

  • References

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