Chapter 4.3. Exploring the Capabilities of Nucleic Acid Polymerases by Use of Directed Evolution

  1. Professor Dr. Carsten Schmuck4 and
  2. Professor Dr. Helma Wennemers5
  1. Susanne Brakmann1,
  2. Marina Schlicke2,
  3. Petra Tafelmeyer3 and
  4. Kai Johnsson3

Published Online: 27 MAY 2005

DOI: 10.1002/3527603727.ch4c

Highlights in Bioorganic Chemistry: Methods and Applications

Highlights in Bioorganic Chemistry: Methods and Applications

How to Cite

Brakmann, S., Schlicke, M., Tafelmeyer, P. and Johnsson, K. (2004) Exploring the Capabilities of Nucleic Acid Polymerases by Use of Directed Evolution, in Highlights in Bioorganic Chemistry: Methods and Applications (eds C. Schmuck and H. Wennemers), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603727.ch4c

Editor Information

  1. 4

    Institute of Organic Chemistry, University of Würzburg, Am Hubland, 97074 Würzburg, Germany

  2. 5

    Department of Chemistry, University of Basel, St. Johanns Ring 19, CH-4056 Basel, Switzerland

Author Information

  1. 1

    Institut für Zoologie, Universität Leipzig, Liebigstraße 18, 04108 Leipzig, Germany

  2. 2

    Universität Leipzig, Biotechnologisch-Biomedizinisches Zentrum, Ritterstraße 16, 04109 Leipzig, Germany

  3. 3

    Swiss Federal Institute of Technology Lausanne (EPFL), Institute of Molecular and Biological Chemistry, Institute of Biomolecular Sciences, 1015 Lausanne, Switzerland

Publication History

  1. Published Online: 27 MAY 2005
  2. Published Print: 14 JAN 2004

ISBN Information

Print ISBN: 9783527306565

Online ISBN: 9783527603725

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Keywords:

  • studies in diagnostic developments;
  • capabilities of nucleic acid polymerases;
  • directed molecular evolution of proteins

Summary

This chapter contains sections titled:

    • Introduction

    • Directed Evolution of Nucleic Acid Polymerases

    • Practical Approaches to the Directed Evolution of Polymerase Function: Selection or Screening?

      • Selection of Polymerases with Altered Activity and Fidelity

      • Screening Polymerase Libraries for Altered Activity

    • Genetic Selection of an Error-prone Variant of Bacteriophage T7 RNA Polymerase

    • Screening for Polymerases with Altered Substrate Tolerance

    • Alternative Scenarios for Assaying Polymerase Activity

    • Concluding Remarks

    • References

    • Directed Molecular Evolution of Proteins