Chapter 10. A Synergy of Technologies: Using Green Fluorescent Protein Tagging and Laser Microsurgery to Study Centrosome Function and Duplication in Vertebrates

  1. Prof. Dr. Erich A. Nigg
  1. Alexey Khodjakov and
  2. Conly L. Rieder

Published Online: 17 JUN 2005

DOI: 10.1002/3527603808.ch10

Centrosomes in Development and Disease

Centrosomes in Development and Disease

How to Cite

Khodjakov, A. and Rieder, C. L. (2004) A Synergy of Technologies: Using Green Fluorescent Protein Tagging and Laser Microsurgery to Study Centrosome Function and Duplication in Vertebrates, in Centrosomes in Development and Disease (ed E. A. Nigg), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603808.ch10

Editor Information

  1. Max-Planck-Institute of Biochemistry, Department of Cell Biology, Am Klopferspitz 18a, 82152 Martinsried, Germany

Author Information

  1. Wadsworth Center, New York State Department of Health, P. O. Box 509, Albany, NY 12201-0509, USA

Publication History

  1. Published Online: 17 JUN 2005
  2. Published Print: 27 JUL 2004

ISBN Information

Print ISBN: 9783527309801

Online ISBN: 9783527603800

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Keywords:

  • centrosomes in development;
  • centrosomes in disease;
  • fluorescent protein tagging;
  • laser microsurgery;
  • centrosome function;
  • centrosome duplication;
  • vertebrates;
  • cell division;
  • spindle assembly;
  • cytokinesis;
  • cell cycle;
  • de novo centrosome formation

Summary

This chapter contains sections titled:

  • Introduction

  • Laser Microsurgery

    • A Brief History of Development

    • Utility for Removing the Centrosome

  • Roles of the Centrosome during Cell Division

    • Role of the Centrosome during Spindle Assembly

    • Role of the Centrosome during Cytokinesis

  • The Centrosome in the Cell Cycle

    • Role of the Centrosome in Progression through the Cell Cycle

    • De Novo Centrosome Formation

  • For the Future

  • Acknowledgments

  • References