Chapter 10. Functional Reconstitution of Specific Porins

  1. Prof. Dr. Roland Benz
  1. Roland Benz and
  2. Frank Orlik

Published Online: 21 JUN 2005

DOI: 10.1002/3527603875.ch10

Bacterial and Eukaryotic Porins: Structure, Function, Mechanism

Bacterial and Eukaryotic Porins: Structure, Function, Mechanism

How to Cite

Benz, R. and Orlik, F. (2004) Functional Reconstitution of Specific Porins, in Bacterial and Eukaryotic Porins: Structure, Function, Mechanism (ed R. Benz), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603875.ch10

Editor Information

  1. Lehrstuhl für Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, 97074 Würzburg, Germany

Author Information

  1. Lehrstuhl für Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, 97074 Würzburg, Germany

Publication History

  1. Published Online: 21 JUN 2005
  2. Published Print: 27 JUL 2004

ISBN Information

Print ISBN: 9783527307753

Online ISBN: 9783527603879

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Keywords:

  • functional reconstitution of specific porins;
  • isolations and purification of specific porins;
  • substrate transport through specific porin channels;
  • carbohydrate binding to the specific porins of the LamB family;
  • LamB of E. coli;
  • CymA of Klebsiella oxytoca;
  • OmpP2 of Haemophilus influenza

Summary

This chapter contains sections titled:

  • Introduction

  • Isolation and Purification of Specific Porins

  • Reconstitution of Specific Porins in Lipid Bilayer Membranes

  • Analysis of Substrate Transport through Specific Porin Channels

    • Study of Ion Transport through the Phosphate-specific OprP of P. aeruginosa

    • Evaluation of the Stability Constant for Binding of Neutral Solutes to the Binding Site inside Specific Porins

    • Investigation of Substrate-binding Kinetics using the Analysis of Current Fluctuations

  • Study of Carbohydrate Binding to the Specific Porins of the LamB Family

    • LamB of E. coli

      • Study of LamB (Maltoporin) Mutants

    • ScrY (Sucrose Porin) of Enteric Bacteria

      • Study of Carbohydrate Binding to ScrY Mutants

  • Properties of the Cyclodextrin (CD)-specific Outer Membrane Porin CymA of Klebsiella oxytoca

  • Porin OmpP2 of Haemophilus influenzae is a Specific Porin for Nicotinamide-derived Nucleotide Substrates

  • Study of the Nucleoside-specific Tsx of E. coli

  • Conclusions