Chapter 8. Drug Efflux and Protein Export through Channel-Tunnels

  1. Prof. Dr. Roland Benz
  1. Christian Andersen

Published Online: 21 JUN 2005

DOI: 10.1002/3527603875.ch8

Bacterial and Eukaryotic Porins: Structure, Function, Mechanism

Bacterial and Eukaryotic Porins: Structure, Function, Mechanism

How to Cite

Andersen, C. (2004) Drug Efflux and Protein Export through Channel-Tunnels, in Bacterial and Eukaryotic Porins: Structure, Function, Mechanism (ed R. Benz), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527603875.ch8

Editor Information

  1. Lehrstuhl für Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, 97074 Würzburg, Germany

Author Information

  1. Department of Biotechnology, Würzburg University Biocenter, Am Hubland, 97074 Würzburg, Germany

Publication History

  1. Published Online: 21 JUN 2005
  2. Published Print: 27 JUL 2004

ISBN Information

Print ISBN: 9783527307753

Online ISBN: 9783527603879

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Keywords:

  • drug efflux;
  • protein transport;
  • channel-tunnels;
  • channel-tunnel-dependent export systems;
  • type I secretion system;
  • TolC

Summary

This chapter contains sections titled:

  • Introduction

  • Channel-tunnel-dependent Export Systems

    • The Type I Secretion System

      • Substrates

      • The Inner Membrane Transporters of Type I Secretion Systems

      • The Accessory Protein of the Type I Secretion System

    • Efflux Pumps

      • The Inner Membrane Transporters of Channel-tunnel-dependent Efflux Pumps

      • Accessory Proteins of Multidrug Efflux Pumps

    • Comparison of Channel-tunnel-dependent Export Systems in E. coli and P. aeruginosa

  • Channel-Tunnels

    • The Structure of TolC

      • The Channel Domain

      • The Tunnel Domain

    • Electrophysiological Characterization of TolC

      • The Role of Aspartate Residues at the Periplasmic Entrance

      • Opening of the Periplasmic Entrance

  • Model for TolC-dependent Export

    • The Role of the Accessory Protein

    • The Mechanism of Protein Secretion

    • The Mechanism of Efflux Pumps

      • Substrate Binding of Different Transporters

      • Export of Substances by RND Transporters Exclusively from the Periplasm?

  • Conclusion