Chapter 2. RNAi in Caenorhabditis elegans

  1. Dr. Ute Schepers

Published Online: 2 AUG 2005

DOI: 10.1002/3527604391.ch2

Book Title

RNA Interference in Practice

RNA Interference in Practice

Additional Information

How to Cite

Schepers, U. (2005) RNAi in Caenorhabditis elegans, in RNA Interference in Practice, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527604391.ch2

Author Information

  1. University of Bonn, Kekulé Institute for Organic Chemistry and Biochemistry, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany

Publication History

  1. Published Online: 2 AUG 2005
  2. Published Print: 25 OCT 2004

ISBN Information

Print ISBN: 9783527310203

Online ISBN: 9783527604395

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Keywords:

  • RNA interference in practice;
  • gene silencing in C. elegans;
  • gene silencing in Drosophila;
  • gene silencing in mammals, RNAi in Caenorhabditis elegans;
  • dsRNA synthesis;
  • mounting animals for microscopy;
  • genome wide screens;
  • C. elegans research;
  • C. elegans webpages

Summary

This chapter contains sections titled:

  • Introduction

  • Application of RNAi in C. elegans

  • Target Sequence Evaluation

  • dsRNA Synthesis

    • Generation of the DNA Template

      • Plasmid Templates

      • DNA Templates Derived by PCR/RT-PCR

    • In-vitro dsRNA Transcription

  • Delivery of dsRNA

    • General Information on the C. elegans Anatomy

    • C. elegans Strains for Silencing

    • Culturing the Worms

    • Microinjection Protocol

    • Soaking Protocol

    • RNAi Feeding Protocol

    • DNA Templates for dsRNA Expression in Feeding E. coli

    • DNA Templates for Hairpin RNA Expression

      • C. elegans Promoters

      • Inverted Repeat Constructs

  • Mounting Animals for Microscopy

  • Genome Wide Screens

    • C. elegans RNAi Library

  • Selected Literature on C. elegans Research

  • Useful C. elegans webpages

  • References

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