Chapter 3. RNAi in Drosophila

  1. Dr. Ute Schepers

Published Online: 2 AUG 2005

DOI: 10.1002/3527604391.ch3

Book Title

RNA Interference in Practice

RNA Interference in Practice

Additional Information

How to Cite

Schepers, U. (2005) RNAi in Drosophila, in RNA Interference in Practice, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527604391.ch3

Author Information

  1. University of Bonn, Kekulé Institute for Organic Chemistry and Biochemistry, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany

Publication History

  1. Published Online: 2 AUG 2005
  2. Published Print: 25 OCT 2004

ISBN Information

Print ISBN: 9783527310203

Online ISBN: 9783527604395

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Keywords:

  • RNA interference in practice;
  • gene silencing in C. elegans;
  • gene silencing in Drosophila;
  • gene silencing in mammals;
  • application of RNAi in Drosophila;
  • dsRNA synthesis;
  • injections;
  • cell lines;
  • RNAi in S2 cells;
  • webpages for Drosophila research

Summary

This chapter contains sections titled:

  • Introduction

  • Application of RNAi in Drosophila

    • dsRNA from Linear DNA Templates

    • dsRNA from Inverted Repeat DNA

    • Inducible Expression in Drosophila Cell Lines

    • Limitations

  • dsRNA Synthesis

    • In-vitro dsRNA Transcription

    • Inverted Repeat DNA

  • Injections

    • Injection Services

    • Injection Method

    • DsRNA or Inverted Repeat DNA Preparation

    • Embryo Collection

  • Cell Lines

  • Protocols

    • Thawing and Maintenance of S2 Cells

    • Freezing Protocol

  • RNAi in S2 Cells

    • dsRNA Transfection Using the Calcium Phosphate Method

    • dsRNA Soaking of S2 Cells

  • High-Throughput Screens

    • Drosophila RNAi Library

  • Useful Webpages for Drosophila Research

  • Books and Literature on Drosophila

  • References

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