Chapter 1. Functional Phases in Cell Attachment and Spreading

  1. Prof. Dr. Doris Wedlich
  1. Michael P. Sheetz,
  2. Benjamin J. Dubin-Thaler,
  3. Gregory Giannone,
  4. Guoying Jiang and
  5. Hans-Günther Döbereiner

Published Online: 9 AUG 2005

DOI: 10.1002/3527604669.ch1

Cell Migration in Development and Disease

Cell Migration in Development and Disease

How to Cite

Sheetz, M. P., Dubin-Thaler, B. J., Giannone, G., Jiang, G. and Döbereiner, H.-G. (2004) Functional Phases in Cell Attachment and Spreading, in Cell Migration in Development and Disease (ed D. Wedlich), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527604669.ch1

Editor Information

  1. University of Karlsruhe, Institute of Zoology II, Kaiserstr. 12, 76131 Karlsruhe, Germany

Author Information

  1. Columbia University, Department of Biological Sciences, Sherman Fairchild Center, 1212 Amsterdam Ave., New York, NY 10027, USA

Publication History

  1. Published Online: 9 AUG 2005
  2. Published Print: 14 DEC 2004

ISBN Information

Print ISBN: 9783527305872

Online ISBN: 9783527604661



  • cell migration;
  • functional phases in cell attachment and spreading;
  • fibroblast spreading on matrices;
  • spreading processes;
  • cell spreading


This chapter contains sections titled:

  • Introduction

  • Fibroblast Spreading on Matrices

  • Summary of Spreading Process

    • Steps in Cell Spreading

      • Basal Motility Phase (Cells in Suspension)

      • Adhesion to the Surface

      • Initiation of Actin Assembly and Spreading (Rate-limiting Step)

      • Continued Spreading

      • Transition to Fully Spread State

    • Binding to Rigid Matrices Causes Strengthening of Cytoskeleton–Integrin Linkages

      • Initial Binding of Fibronectin Multimers at the Leading Edge and Over Actin Cables

      • Force-dependent Activation of the ECM–Integrin Complexes

      • Additional Steps in the Spreading Process

    • MTs and Motility

    • Conclusion

  • References