Chapter 10. Rotation of F1-ATPase

  1. Masamitsu Futai2,
  2. Yoh Wada3 and
  3. Jack H. Kaplan4
  1. Eiro Muneyuki and
  2. Masasuke Yoshida

Published Online: 6 DEC 2005

DOI: 10.1002/3527606122.ch10

Handbook of ATPases: Biochemistry, Cell Biology, Pathophysiology

Handbook of ATPases: Biochemistry, Cell Biology, Pathophysiology

How to Cite

Muneyuki, E. and Yoshida, M. (2004) Rotation of F1-ATPase, in Handbook of ATPases: Biochemistry, Cell Biology, Pathophysiology (eds M. Futai, Y. Wada and J. H. Kaplan), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527606122.ch10

Editor Information

  1. 2

    Division of Biological Sciences, Nanoscience and Nanotechnology Center, Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan

  2. 3

    Division of Biological Sciences, Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan

  3. 4

    University of Illinois at Chicago, Department of Biochemistry and Molecular Genetics, 900 S. Ashland Ave., Chicago, IL 60607, USA

Author Information

  1. Research Laboratory of Resources Utiliziation, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan

Publication History

  1. Published Online: 6 DEC 2005
  2. Published Print: 24 JUN 2004

ISBN Information

Print ISBN: 9783527306893

Online ISBN: 9783527606122

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Keywords:

  • ATPases;
  • F-type ATPases;
  • rotation of F1-ATPase;
  • biochemical characterization;
  • structural study;
  • rotation

Summary

This chapter contains sections titled:

  • Abbreviations

  • Introduction

  • Biochemical Characterization

    • Oxygen Exchange Reaction and Boyer's Binding Change Mechanism

    • Rotary Catalysis Hypothesis by Boyer

  • Structural Study

    • “Native” Structure Revealed

    • (ADP·AlF4)2F1 Structure Revealed

  • Rotation

    • Rotation of γ Subunit Observed at Video Rate

    • Effect of Nucleotide Binding on Torque Generation

    • Resolution of the Continuous Rotation into 120° Steps and into Substeps

    • Direct Observation of ATPMg Inhibition at the Single Molecule Level

    • Effects of Amino Acid Substitutions on the Rotation

  • Mechanistic Implication and Possible Mechanism

    • Problems in ATPase Catalytic Scheme

    • Correlation Between Crystal Structures and Rotation Intermediates

    • Correlation Between ATPase Catalytic Intermediates and Rotation Intermediates

    • Possible Working Hypotheses

    • Model Assuming the Native Structure is in the 0° Dwell

    • Model Assuming the Native Structure is in the 90° Dwell

  • Epilogue

  • Acknowledgments

  • References