Chapter 18. Lytic Function of Vacuole, Molecular Dissection of Autophagy in Yeast

  1. Masamitsu Futai2,
  2. Yoh Wada3 and
  3. Jack H. Kaplan4
  1. Yoshinori Ohsumi

Published Online: 6 DEC 2005

DOI: 10.1002/3527606122.ch18

Handbook of ATPases: Biochemistry, Cell Biology, Pathophysiology

Handbook of ATPases: Biochemistry, Cell Biology, Pathophysiology

How to Cite

Ohsumi, Y. (2004) Lytic Function of Vacuole, Molecular Dissection of Autophagy in Yeast, in Handbook of ATPases: Biochemistry, Cell Biology, Pathophysiology (eds M. Futai, Y. Wada and J. H. Kaplan), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527606122.ch18

Editor Information

  1. 2

    Division of Biological Sciences, Nanoscience and Nanotechnology Center, Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan

  2. 3

    Division of Biological Sciences, Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan

  3. 4

    University of Illinois at Chicago, Department of Biochemistry and Molecular Genetics, 900 S. Ashland Ave., Chicago, IL 60607, USA

Author Information

  1. Department of Cell Biology, National Institute for Basic Biology, Nishigonaka 38, Myodaijicho, Okazaki 444-8585, Japan

Publication History

  1. Published Online: 6 DEC 2005
  2. Published Print: 24 JUN 2004

ISBN Information

Print ISBN: 9783527306893

Online ISBN: 9783527606122

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Keywords:

  • ATPases;
  • cell biology;
  • pathophysiology of ATPases;
  • compartments;
  • lytic function of vacuole;
  • molecular dissection of autophagy in yeast;
  • genetic approaches to yeast autophagy;
  • proteins involved in autophagy;
  • function of the Apg proteins;
  • disintegration of autophagic bodies;
  • physiological roles of autophagy;
  • autophagy in higher eukaryotes

Summary

This chapter contains sections titled:

  • Introduction

  • Vacuolar Protein Degradation in Yeast

  • Intracellular Bulk Degradation – Autophagy

  • Discovery of Autophagy in Yeast

  • Monitoring of Autophagy

  • Genetic Approaches to Yeast Autophagy

  • Characterization of Autophagy Defective Mutants

  • Proteins Involved in Autophagy

    • Apg12 Conjugation System

    • Apg8 Lipidation System

    • Apg1 Protein Kinase Complex

    • PI 3-kinase Complex

  • Function of the Apg Proteins

  • Disintegration of Autophagic Bodies in the Vacuole

  • Physiological Roles of Autophagy

  • Autophagy in Higher Eukaryotes

  • References