Chapter 3. Catalytic and Transport Mechanism of the Sarco-(Endo)Plasmic Reticulum Ca2+-ATPase (SERCA)

  1. Masamitsu Futai3,
  2. Yoh Wada4 and
  3. Jack H. Kaplan5
  1. Giuseppe Inesi1 and
  2. Chikashi Toyoshima2

Published Online: 6 DEC 2005

DOI: 10.1002/3527606122.ch3

Handbook of ATPases: Biochemistry, Cell Biology, Pathophysiology

Handbook of ATPases: Biochemistry, Cell Biology, Pathophysiology

How to Cite

Inesi, G. and Toyoshima, C. (2004) Catalytic and Transport Mechanism of the Sarco-(Endo)Plasmic Reticulum Ca2+-ATPase (SERCA), in Handbook of ATPases: Biochemistry, Cell Biology, Pathophysiology (eds M. Futai, Y. Wada and J. H. Kaplan), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527606122.ch3

Editor Information

  1. 3

    Division of Biological Sciences, Nanoscience and Nanotechnology Center, Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan

  2. 4

    Division of Biological Sciences, Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan

  3. 5

    University of Illinois at Chicago, Department of Biochemistry and Molecular Genetics, 900 S. Ashland Ave., Chicago, IL 60607, USA

Author Information

  1. 1

    Department of Biochemistry, University of Maryland School of Medicine, 108 N. Green Street, Baltimore, MD 21201, USA

  2. 2

    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan

Publication History

  1. Published Online: 6 DEC 2005
  2. Published Print: 24 JUN 2004

ISBN Information

Print ISBN: 9783527306893

Online ISBN: 9783527606122

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Keywords:

  • ATPases;
  • P-type ATPases;
  • catalytic mechanism;
  • transport mechanism;
  • sarco-(endo)plasmic reticulum Ca2+-ATPase (SERCA);
  • functional characterization;
  • structural characterization;
  • binding of ligands;
  • conformational changes

Summary

This chapter contains sections titled:

  • Summary

  • Introduction

  • Experimental Systems

  • Functional Characterization

  • Structural Characterization

    • Extramembranous Region and the Catalytic Domains of E1·2Ca2+

    • Transmembrane region of E1·2Ca2+

    • Enzyme Structure in the Absence of Ca2+ (E2·TG)

    • Thapsigargin-binding Domain

    • Interaction with Phospholamban

  • Binding of Ligands, Catalytic Events and Conformational Changes

    • Ca2+ Binding and Catalytic Activation

    • Nucleotide Binding and Substrate-induced Conformational Fit

    • Phosphoryl Transfer and Hydrolytic Cleavage

    • Interconversion of Phosphorylation and Ca2+ Binding Potentials

  • References