Chapter 7. Artificial Restriction Enzymes As Tools for Future Molecular Biology and Biotechnology

  1. Prof. Dr. Ronald Breslow
  1. Yoji Yamamoto and
  2. Makoto Komiyama

Published Online: 24 MAR 2006

DOI: 10.1002/3527606645.ch7

Artificial Enzymes

Artificial Enzymes

How to Cite

Yamamoto, Y. and Komiyama, M. (2005) Artificial Restriction Enzymes As Tools for Future Molecular Biology and Biotechnology, in Artificial Enzymes (ed R. Breslow), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527606645.ch7

Editor Information

  1. Columbia University, Department of Chemistry, 556 Chandler Laboratory, New York, NY 10027-6948, USA

Author Information

  1. The University of Tokyo, Research Center for Advanced Science and Technology, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan

Publication History

  1. Published Online: 24 MAR 2006
  2. Published Print: 25 MAY 2005

ISBN Information

Print ISBN: 9783527311651

Online ISBN: 9783527606641



  • artificial enzymes;
  • artificial restriction enzymes;
  • tools;
  • molecular biology;
  • biotechnology;
  • non-enzymatic catalysts;
  • DNA hydrolysis;
  • molecular design;
  • site-selective scission;
  • single-stranded DNA;
  • double-stranded DNA


This chapter contains sections titled:

  • Introduction

  • Significance of Artificial Restriction Enzymes

  • Non-enzymatic Catalysts for DNA Hydrolysis

  • Molecular Design of Artificial Restriction Enzymes (Covalent vs. Non-Covalent Strategy)

    • Covalent Strategy for the First-generation of Artificial Restriction Enzymes

    • Non-covalent Strategy for the Second-generation of Artificial Restriction Enzymes

    • Chemical Basis for “Non-covalent” Strategy

  • Site-selective Scission of Single-stranded DNA

    • Promotion of Gap-selective DNA Hydrolysis by Introducing Monophosphate Groups to the Gap-site

    • Enzymatic Ligation of the Fragments Obtained by Site-selective Scission

  • Site-selective Scission of Double-stranded DNA by Combining Ce(IV)/EDTA Complex with Pseudo-complementary PNA

    • Design of Artificial Restriction Enzymes for Double-stranded DNA Scission

    • Site-selective Hydrolysis of Double-stranded DNA

    • Enzymatic Ligation of the Scission Fragment and Foreign DNA

  • Conclusion