Chapter 13. Protein–Lipid Interactions in the Formation of Raft Microdomains in Biological Membranes

  1. Prof. Dr. Lukas K. Tamm
  1. Prof. Akihiro Kusumi1,
  2. Prof. Kenichi Suzuki1,
  3. Junko Kondo1,
  4. Nobuhiro Morone2,
  5. Yasuhiro Umemura1

Published Online: 29 MAR 2006

DOI: 10.1002/3527606769.ch13

Book Title

Protein-Lipid Interactions: From Membrane Domains to Cellular Networks

Protein-Lipid Interactions: From Membrane Domains to Cellular Networks

Additional Information

How to Cite

Kusumi, A., Suzuki, K., Kondo, J., Morone, N. and Umemura, Y. (2006) Protein–Lipid Interactions in the Formation of Raft Microdomains in Biological Membranes, in Protein-Lipid Interactions: From Membrane Domains to Cellular Networks (ed L. K. Tamm), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527606769.ch13

Editor Information

  1. Professor of Molecular Physiology and Biological Physics, University of Virginia, PO Box 800736, Charlottesville, VA 22908-0736, USA

Author Information

  1. 1

    Institute for Frontier Medicine, Kyoto University, Shougoin, Sakyo-ku, Kyoto 606-8507, Japan

  2. 2

    Department of Ultrastructural Research, National Center of Neurology and Psychiatry, Kodaira, Tokyo 187-8502, Japan

Publication History

  1. Published Online: 29 MAR 2006
  2. Published Print: 5 AUG 2005

ISBN Information

Print ISBN: 9783527311514

Online ISBN: 9783527606764

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Keywords:

  • protein–lipid interactions in the formation of raft microdomains;
  • molecular complexes;
  • microdomains;
  • membrane skeleton-based compartments;
  • raft domains;
  • raft hypothesis

Summary

This chapter contains sections titled:

  • Many Plasma Membrane Functions are Mediated by Molecular Complexes, Microdomains and Membrane Skeleton-based Compartments

  • Timescales, Please!

  • Four Types of Membrane Domains

  • The Cell Membrane is a Two-dimensional Non-ideal Liquid Containing Dynamic Structures on Various Time-Space Scales

  • A Definition of Raft Domains

  • The Original Raft Hypothesis

  • Are there Raft Domains in Steady-state Cells in the Absence of Extracellular Stimulation?

    • Standard Immunofluorescence or Immunoelectron Microscopy Failed to Detect Raft-like Domains in the Plasma Membrane of Steady-state Cells

    • The Recovery of a Molecule in Detergent-resistant Membrane (DRM) Fractions Might Infer its Raft Association in the Cell Membrane, but the Relationship between DRM Fractions and Raft Domains is Complicated

    • The Size of Rafts in Plasma Membranes of Steady-state Cells may be 10 nm or Less

    • Mushroom Model for the Steady-state Raft

  • Stabilized Rafts Induced by Protein Clustering in Plasma and Golgi Membranes

    • Clustering of Raft Molecules by Ligand Binding or Crosslinking Induces Stabilized Rafts (“Receptor-cluster Rafts”)

    • How can Raft Molecule Clustering Induce Stabilized Rafts?

  • Can Receptor-cluster Rafts Work as Platforms to Facilitate the Assembly of Raftophilic Molecules?

    • Benchmarks for Experiments Examining the Colocalization of Raftophilic Molecules

    • Simultaneous Crosslinking of Two GPI-anchored Receptors

    • Sequential Crosslinking of One Species of GPI-anchored Receptors Followed by Crosslinking of a Second Species without Fixation

    • Examination of the Recruitment of Non-crosslinked Second Raftophilic Molecules to Crosslinked GPI-anchored Receptor Clusters

    • Difficulty in Colocalization Experiments using Raftophilic Molecules: Low Levels of Colocalization and Quantitative Reproducibility Due to Sensitivity to Subtle Differences in Experimental Conditions and Protocols

  • Timescales Again! Transient Colocalization of Raftophilic Molecules

  • Modified Raft Hypothesis

  • References

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